Development of redox-labeled electrochemical immunoassay for polycyclic aromatic hydrocarbons with controlled surface modification and catalytic voltammetric detection
文献类型：期刊论文
| 作者 | Wei, Ming-Yuan ; Wen, Shou-Dong ; Yang, Xi-Qiang ; Guo, Liang-Hong |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2009-05-15 |
| 卷号 | 24期号:9页码:2909-2914 |
| 关键词 | Electrochemical immunoassay Catalytic voltammetry Polycyclic aromatic hydrocarbons Surface modification Indium tin oxide |
| 中文摘要 | A redox-labeled direct competitive electrochemical immunoassay for polycyclic aromatic hydrocarbons (PAHs) was developed. A ruthenium tris(bipyridine)-pyrenebutyric acid conjugate was synthesized as the redox-labeled tracer. Its recognition by an anti-PAH monoclonal antibody was confirmed by surface plasmon resonance. In the immunoassay, the antibody was immobilized on (3-glycidoxypropyl)-trimethoxysilane (GPTMS)-modified indium tin oxide (ITO) electrodes. The assay was quantified by measuring the electro-catalytic current of the redox label in an oxalate-containing electrolyte which served as a sacrificial electron donor to amplify the current signal. Formation of GPTMS film on ITO and subsequent antibody immobilization were characterized by X-ray photoelectron spectroscopy (XPS) and electrochemistry. Using a ruthenium tris(bipyridine)-conjugated IgG (IgG-Ru) as the surface-bound redox probe, the highest electrochemical signal was obtained on GPTMS electrodes with 1 h modification. Under the optimized conditions for ITO modification, antibody immobilization and tracer concentration, competition curves for benzo[a]pyrene and pyrenebutyric acid were obtained with a detection limit of 2.4 and 10 ng mL(-1), respectively. The redox-labeled electrochemical immunoassay with signal amplification mechanism offers a potential analytical method for the simultaneous detection of multiple environmental organic pollutants on antibody biochips. (C) 2009 Elsevier B.V. All rights reserved. |
| WOS记录号 | WOS:000266192900025 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21484]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Wei, Ming-Yuan,Wen, Shou-Dong,Yang, Xi-Qiang,et al. Development of redox-labeled electrochemical immunoassay for polycyclic aromatic hydrocarbons with controlled surface modification and catalytic voltammetric detection[J]. BIOSENSORS & BIOELECTRONICS,2009,24(9):2909-2914. |
| APA | Wei, Ming-Yuan,Wen, Shou-Dong,Yang, Xi-Qiang,&Guo, Liang-Hong.(2009).Development of redox-labeled electrochemical immunoassay for polycyclic aromatic hydrocarbons with controlled surface modification and catalytic voltammetric detection.BIOSENSORS & BIOELECTRONICS,24(9),2909-2914. |
| MLA | Wei, Ming-Yuan,et al."Development of redox-labeled electrochemical immunoassay for polycyclic aromatic hydrocarbons with controlled surface modification and catalytic voltammetric detection".BIOSENSORS & BIOELECTRONICS 24.9(2009):2909-2914. |
入库方式： OAI收割
来源：生态环境研究中心
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