Improved preparation and identification of aristolochic acid-DNA adducts by solid-phase extraction with liquid chromatography-tandem mass spectrometry
文献类型：期刊论文
| 作者 | Gao Haiyan ; Guo Fangqiu ; Feng Feng ; Yin Junfa ; Song Maoyong ; Wang Hailin |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
 |
| 出版日期 | 2009 |
| 卷号 | 21期号:12页码:1769-1776 |
| 关键词 | aristolochic acid DNA adducts solid phase extraction quadrupole time-of-flight tandem mass spectrometry |
| 中文摘要 | Aristolochic acid (AA) is a known nephrotoxin and potential carcinogen, which can form covalent DNA adducts after metabolic activation in vivo and in vitro. A simple method for preparation and characterization of aristolochic acid-DNA adducts was developed. Four AA-adducts were synthesized by a direct reaction of AAI/AAII with 2'-deoxynucleosides. The reaction mixture was first cleaned-up and pre-concentrated using solid phase extraction (SPE), and further purified by a reversed-phase high performance liquid chromatography (HPLC). By the application of developed SPE procedure, matrices and byproducts in reaction mixture could be greatly reduced and adducts of high purity (more than 94% as indicated by HPLC) were obtained. The purified AA-DNA adducts were identified and characterized with liquid-electrospray ionization-quadrupole-time of flight-mass spectrometry (LC-ESI-Q-TOF-MS/MS) and LC-Diode array detector-fluorescence (LC-DAD-FL) analysis. This work provides a robust tool for possible large-scale preparation of AA-DNA adduct standards, which can promote the further studies on carcinogenic and mutagenic mechanism of aristolochic acids. |
| WOS记录号 | WOS:000273080800022 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21517]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Gao Haiyan,Guo Fangqiu,Feng Feng,et al. Improved preparation and identification of aristolochic acid-DNA adducts by solid-phase extraction with liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2009,21(12):1769-1776. |
| APA | Gao Haiyan,Guo Fangqiu,Feng Feng,Yin Junfa,Song Maoyong,&Wang Hailin.(2009).Improved preparation and identification of aristolochic acid-DNA adducts by solid-phase extraction with liquid chromatography-tandem mass spectrometry.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,21(12),1769-1776. |
| MLA | Gao Haiyan,et al."Improved preparation and identification of aristolochic acid-DNA adducts by solid-phase extraction with liquid chromatography-tandem mass spectrometry".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 21.12(2009):1769-1776. |
入库方式： OAI收割
来源：生态环境研究中心
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