One-step and high-density protein immobilization on epoxysilane-modified silica nanoparticles
文献类型：期刊论文
| 作者 | Zhang Qin; Huang RongFu; Guo Liang-Hong |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2009-08 |
| 卷号 | 54期号:15页码:2620-2626 |
| 关键词 | epoxysilane silica nanoparticle protein immobilization |
| 中文摘要 | Silica nanoparticles are most commonly modified with amino-silanes, followed by post-modification activation for protein immobilization. In this work, epoxy-functionalized silica nanoparticles were prepared by modification with glycidyloxypropyl trimethoxysilane (GPTMS) for direct protein immobilization. Silica nanoparticles possessed an average size of 46 nm, but increased to 63 nm after GPTMS modification. Reaction time, reaction temperature and GPTMS content had no significant effect on particle size. Zeta potential of SiO(2) changed from -26mV to +38mV after modification. Fourier-transformed infrared spectroscopy revealed alkyl C-H bending and stretching bands at 2944 cm(-1), 1343 cm(-1) and 1465 cm(-1), respectively, for the modified nanoparticles. Fluorescein cadaverine was found to bind to GPTMS-modified SiO(2), but not to bare SiO(2), indicating the chemical reactivity of epoxy groups on the modified nanoparticle with amines. Finally, fluorescently labeled bovine serum albumin (BSA) was used as a model protein to investigate the capacity of epoxy-SiO(2) nanoparticles for protein immobilization. The results showed that more proteins were immobilized on the particle with longer reaction time, higher NaCl concentration, lower pH, and less GPTMS content. More importantly, proteins bound to epoxy-SiO(2) nanoparticle were highly stable. Under optimized reaction conditions, as much as 25 mg BSA/g nanoparticle was covalently attached to the nanoparticle. The epoxy silane modification of silica nanoparticles offers a reactive surface for one-step and high-density protein immobilization. |
| WOS记录号 | WOS:000268571300009 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21543]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang Qin,Huang RongFu,Guo Liang-Hong. One-step and high-density protein immobilization on epoxysilane-modified silica nanoparticles[J]. CHINESE SCIENCE BULLETIN,2009,54(15):2620-2626. |
| APA | Zhang Qin,Huang RongFu,&Guo Liang-Hong.(2009).One-step and high-density protein immobilization on epoxysilane-modified silica nanoparticles.CHINESE SCIENCE BULLETIN,54(15),2620-2626. |
| MLA | Zhang Qin,et al."One-step and high-density protein immobilization on epoxysilane-modified silica nanoparticles".CHINESE SCIENCE BULLETIN 54.15(2009):2620-2626. |
入库方式： OAI收割
来源：生态环境研究中心
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