Potential Mechanisms Involved in Ceramide-induced Apoptosis in Human Colon Cancer HT29 Cells
文献类型：期刊论文
| 作者 | Wang, Jing ; Lv, Xiao-Wen ; Du, Yu-Guo |
| 刊名 | BIOMEDICAL AND ENVIRONMENTAL SCIENCES
 |
| 出版日期 | 2009-02 |
| 卷号 | 22期号:1页码:76-85 |
| 关键词 | Ceramide HT29 cells Apoptosis Cell signaling p38 MAPK NF kappa B |
| 中文摘要 | Objective To investigate the potential mechanisms of cell death after the treatment with ceramide. Methods MTT assay, DNA ladder, reporter assay, FACS and Western blot assay were employed to investigate the potential mechanisms of cell death after the treatment with C2-ceramide. Results A short-time treatment with C2-ceramide induced cell death. which was associated with p38 MAP kinase activation, but had no links with typical caspase activation or PARP degradation. Rather than caspase inhibitor, Inhibitor of p38 MAP kinase blocked cell death induced by a short-time treatment with ceramide (<12 h). However, inhibition of p38 MAP kinase could not block cell death induced by a prolonged treatment with ceramide (>12 h). Moreover, incubation of cells with ceramide for a long time (>12 h) increased subG1, but reduced S phase accompanied by caspase-dependent and caspase-independent changes including NF kappa B activation. Conclusion Ceramide-induced cell apoptosis involves both caspase-dependent and -independent signaling pathway. Caspase-independent cell death occurring in a relatively early stage, which is mediated via p38 MAP kinase, can progress into a stage involving both caspase-dependent and -independent mechanisms accompanied by cell signaling of MAPKs and NF kappa B. |
| WOS记录号 | WOS:000263776100013 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21556]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Jing,Lv, Xiao-Wen,Du, Yu-Guo. Potential Mechanisms Involved in Ceramide-induced Apoptosis in Human Colon Cancer HT29 Cells[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2009,22(1):76-85. |
| APA | Wang, Jing,Lv, Xiao-Wen,&Du, Yu-Guo.(2009).Potential Mechanisms Involved in Ceramide-induced Apoptosis in Human Colon Cancer HT29 Cells.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,22(1),76-85. |
| MLA | Wang, Jing,et al."Potential Mechanisms Involved in Ceramide-induced Apoptosis in Human Colon Cancer HT29 Cells".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 22.1(2009):76-85. |
入库方式： OAI收割
来源：生态环境研究中心
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