Real-time molecular recognition between protein and photosensitizer of photodynamic therapy by quartz crystal microbalance sensor
文献类型：期刊论文
| 作者 | Yang, Yu ; Long, Yuanyuan ; Li, Zhiyu ; Li, Na ; Li, Kean ; Liu, Feng |
| 刊名 | ANALYTICAL BIOCHEMISTRY
 |
| 出版日期 | 2009-09-01 |
| 卷号 | 392期号:1页码:22-27 |
| 关键词 | Quartz crystal microbalance Sensor Protein Photosensitizer |
| 中文摘要 | Real-time investigation of molecular recognition between protein and the photosensitizer of photodynamic therapy (PDT) was carried out by a quartz crystal microbalance (QCM) sensor integrated into a flow injection analysis (FIA) system. The photosensitizer meso-tetrakis(4-hydroxyphenyl)porphyrin (p-THPP) was immobilized on the gold electrode of the QCM chip by combining the sol-gel and self-assembly methods. Such a rapid screen analysis of molecular recognition showed that the p-THPP-immobilized sensor exhibited sensitive and specific interaction only with hemoglobin (Hb). The kinetic rate constants (k(ass) and k(diss)) and the equilibrium association constant (K(A)) for p-THPP-Hb interaction were calculated by linear regression. The sensing performance characteristics of the proposed sensor were investigated. The sensor showed excellent selectivity, reproducibility, and repeatability for the detection of Hb. A linear calibration plot was obtained over a range from 0.2 to 1.0 mu M with a detection limit (signal/noise ratio = 3) of 0.15 mu M. The response mechanism of the sensor is discussed in detail. Due to its low cost and simple manipulation, this QCM-FIA system was shown to be a highly effective method for the investigation of interaction between biomacromolecules and the PDT photosensitizer. It also provides a potential strategy for screening an efficient and less harmful photosensitizer for PDT application. (c) 2009 Elsevier Inc. All rights reserved. |
| WOS记录号 | WOS:000267920600004 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21562]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Yang, Yu,Long, Yuanyuan,Li, Zhiyu,et al. Real-time molecular recognition between protein and photosensitizer of photodynamic therapy by quartz crystal microbalance sensor[J]. ANALYTICAL BIOCHEMISTRY,2009,392(1):22-27. |
| APA | Yang, Yu,Long, Yuanyuan,Li, Zhiyu,Li, Na,Li, Kean,&Liu, Feng.(2009).Real-time molecular recognition between protein and photosensitizer of photodynamic therapy by quartz crystal microbalance sensor.ANALYTICAL BIOCHEMISTRY,392(1),22-27. |
| MLA | Yang, Yu,et al."Real-time molecular recognition between protein and photosensitizer of photodynamic therapy by quartz crystal microbalance sensor".ANALYTICAL BIOCHEMISTRY 392.1(2009):22-27. |
入库方式： OAI收割
来源：生态环境研究中心
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