Ultra-performance liquid chromatography-tandem mass spectrometry for rapid and highly sensitive analysis of stereoisomers of benzo[a]pyrene diol epoxide-DNA adducts
文献类型：期刊论文
| 作者 | Feng, Feng; Wang, Xiaoli; Yuan, Hancheng; Wang, Hailin |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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| 出版日期 | 2009-07-15 |
| 卷号 | 877期号:22页码:2104-2112 |
| 关键词 | UPLC ESI-MS/MS Benzo[a]pyrene DNA adducts |
| 中文摘要 | An ultra-performance liquid chromatography tandem mass spectrometry with multiple reaction monitoring method (UPLC-MRM/MS) is developed for fast and sensitive analysis of four genotoxic stereoisomers of anti-benzoialpyrene diol epoxide (BPDE)-N(2)dG adducts (trans-(+), trans-(-), cis-(+) and cis-(-)), which result from environmental exposure to ubiquitous pollutant benzo[a]pyrene (B[a]P). The developed method displays a low limit of detection of <0.7fmol (S/N=3) for the four stereoisomers of anti-BPDE-N(2)dG, a dynamic range of 2 orders of magnitude (2.3-630 fmol, R(2) >= 0.997), and one separation of 2-4 min. The developed method enables us to use the stereoisomers; of anti-BPDE-N(2)dG as a biomarker and to study the stereoselectivity of metabolic activation of B[a]P in human lung A549 cells. The UPLC-MRM/MS analysis of cellular DNA exposed to B[a]P show that activation of B[a]P in A549 cells predominantly induces trans-(+)-anti-BPDE-N(2)dG with cis-(+)-anti-BPDE-N(2)dG and one syn-BPDE-N(2)dG as two minorities, while trans-(-)-anti-BPDE-N(2)dG and cis-(-)-anti-BPDE-N(2)dG are absent. The observed preferential formation of trans-(+)-anti-BPDE-N(2)dG in B[a]P treated A549 cells may result from combined stereoselectivity of the metabolic activation of B[a]P and the reaction of anti-BPDE with dsDNA. The results also suggest that a number of key optical intermediates are formed during activation of B[a]P in A549 cells, including t-rans-(+)-B[a]P-7,8-dihydrodiol and trans-(-)-B[a]P-7,8-dihydrodiol and their corresponding downstream metabolites (+)-anti-BPDE and (+)-syn-BPDE. (C) 2009 Elsevier B.V. All rights reserved. |
| WOS记录号 | WOS:000268040400018 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21585]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Feng, Feng,Wang, Xiaoli,Yuan, Hancheng,et al. Ultra-performance liquid chromatography-tandem mass spectrometry for rapid and highly sensitive analysis of stereoisomers of benzo[a]pyrene diol epoxide-DNA adducts[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2009,877(22):2104-2112. |
| APA | Feng, Feng,Wang, Xiaoli,Yuan, Hancheng,&Wang, Hailin.(2009).Ultra-performance liquid chromatography-tandem mass spectrometry for rapid and highly sensitive analysis of stereoisomers of benzo[a]pyrene diol epoxide-DNA adducts.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,877(22),2104-2112. |
| MLA | Feng, Feng,et al."Ultra-performance liquid chromatography-tandem mass spectrometry for rapid and highly sensitive analysis of stereoisomers of benzo[a]pyrene diol epoxide-DNA adducts".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 877.22(2009):2104-2112. |
入库方式： OAI收割
来源：生态环境研究中心
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