On-line liquid chromatography-mass spectrometry with dilution line to achieve large volume urine injection for the improvement of sensitivity
文献类型：期刊论文
| 作者 | Liu, Min; Yan, Wei; Lin, Jin-Ming; Hashi, Yuki; Liu, Li-bin; Wei, Yanlin |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2008-07-11 |
| 卷号 | 1198期号:1页码:87-94 |
| 关键词 | large volume injection dilution line on-line liquid chromatography-mass spectrometry estrogens spiked urine samples |
| 英文摘要 | A unique automated on-line high-performance liquid chromatography-mass spectrometry (LC-MS) method was developed allowing injection of a large volume of urine for the improvement of sensitivity using estrogens as analytes. The urine sample was precipitated by acetonitrile (sample: acetonitrile, 1 :2, v/v) to remove most proteins. Then the supernatant was directly delivered at 0.3 mL/min by a pump, which broke through the limit of autosampler with fixed loop. Then the sample was loaded onto a restricted access media (RAM) pre-column after on-line dilution with NH4Ac solution which was delivered by another pump under high flow-rate. After the addition of on-line dilution line, the content of organic solvent in the sample aliquot was sharply decreased so that the analytes were effectively trapped by pre-column. In addition, another 6-port valve was added to selectively deliver the effluent containing analytes into MS, which minimized the pollution of the MS and matrix effect. After optimization of the condition of extraction method, 3 mL sample (containing I mL urine) could be successfully enriched. The injection volume increased 10 times compared with conventional on-line LC-MS methods for biofluid analysis, significantly improving sensitivity. Moreover, no obvious increase of column pressure was observed after 300 injections. The method was validated by spiking urine. Linearity was determined by sample which was in the range of 1-500 ng/mL. The process efficiency ranged from 70.2 to 106% with RSDs less than 15% with the exception of a few analytes. The limit of detection (LOD) and quantification (LOQ) ranged from 0.3 to 2 ng/mL and 1 to 8 ng/mL, respectively, corresponding a decrease of 1-2 orders of magnitude compared with those of off-line and on-line methods. (c) 2008 Elsevier B.V. All rights reserved. |
| WOS记录号 | WOS:000257545600014 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21966]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Liu, Min,Yan, Wei,Lin, Jin-Ming,et al. On-line liquid chromatography-mass spectrometry with dilution line to achieve large volume urine injection for the improvement of sensitivity[J]. JOURNAL OF CHROMATOGRAPHY A,2008,1198(1):87-94. |
| APA | Liu, Min,Yan, Wei,Lin, Jin-Ming,Hashi, Yuki,Liu, Li-bin,&Wei, Yanlin.(2008).On-line liquid chromatography-mass spectrometry with dilution line to achieve large volume urine injection for the improvement of sensitivity.JOURNAL OF CHROMATOGRAPHY A,1198(1),87-94. |
| MLA | Liu, Min,et al."On-line liquid chromatography-mass spectrometry with dilution line to achieve large volume urine injection for the improvement of sensitivity".JOURNAL OF CHROMATOGRAPHY A 1198.1(2008):87-94. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。