Photoelectrochemical sensor for the rapid detection of in situ DNA damage induced by enzyme-catalyzed Fenton reaction
文献类型：期刊论文
| 作者 | Liang, Minmin ; Jia, Suping ; Zhu, Shengchao ; Guo, Liang-Hong |
| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2008-01-15 |
| 卷号 | 42期号:2页码:635-639 |
| 英文摘要 | Photoelectrochemical sensors were developed for the rapid detection of oxidative DNA damage induced by Fe2+ and H2O2 generated in situ by the enzyme glucose oxidase. The sensor is a multilayer film prepared on a tin oxide nanoparticle electrode by layer-by-layer self-assembly and is composed of separate layers of a photoelectrochemical indicator, DNA, and glucose oxidase. The enzyme catalyzes the formation of H2O2 in the presence of glucose, which then reacts with Fe2+ and generates hydroxyl radicals by the Fenton reaction. The radicals attack DNA in the sensor film, mimicking metal toxicity pathways in vivo. The DNA damage is detected by monitoring the change of photocurrent of the indicator. In one sensor configuration, a DNA intercalator, Ru(bpy)(2)(dppz)(2+) (bpy = 2,2'-bipyridine, dppz = dipyrido[3,2-a:2',3'-c]phenazine), was employed as the photoelectrochemical indicator. The damaged DNA on the sensor bound less Ru(bpy)(2)(dppz)(2+) than the intact DNA, resulting in a drop in photocurrent. In another configuration, ruthenium tris(bipyridine) was used as the indicator and was immobilized on the electrode underneath the DNA layer. After oxidative damage, the DNA bases became more accessible to photoelectrochemical oxidation than the intact DNA, producing a rise in photocurrent. Both sensors displayed substantial photocurrent change after incubation in Fe2+/glucose in a time-dependent manner. And the detection limit of the first sensor was less than 50 mu M. The results were verified independently by fluorescence and gel electrophoresis experiments. When fully integrated with cell-mimicking components, the photoelectrochemical DNA sensor has the potential to become a rapid, high-throughput, and inexpensive screening tool for chemical genotoxicity. |
| WOS记录号 | WOS:000252317700054 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21975]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Liang, Minmin,Jia, Suping,Zhu, Shengchao,et al. Photoelectrochemical sensor for the rapid detection of in situ DNA damage induced by enzyme-catalyzed Fenton reaction[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2008,42(2):635-639. |
| APA | Liang, Minmin,Jia, Suping,Zhu, Shengchao,&Guo, Liang-Hong.(2008).Photoelectrochemical sensor for the rapid detection of in situ DNA damage induced by enzyme-catalyzed Fenton reaction.ENVIRONMENTAL SCIENCE & TECHNOLOGY,42(2),635-639. |
| MLA | Liang, Minmin,et al."Photoelectrochemical sensor for the rapid detection of in situ DNA damage induced by enzyme-catalyzed Fenton reaction".ENVIRONMENTAL SCIENCE & TECHNOLOGY 42.2(2008):635-639. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。