Identification of Retinoic Acid Receptor Agonists in Sewage Treatment Plants
文献类型：期刊论文
| 作者 | Zhen, Huajun ; Wu, Xiaoqin ; Hu, Jianying ; Xiao, Yang ; Yang, Min ; Hirotsuji, Junji ; Nishikawa, Jun-ichi ; Nakanishi, Tsuyoshi ; Ike, Michihiko |
| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2009-09-01 |
| 卷号 | 43期号:17页码:6611-6616 |
| 中文摘要 | Retinoic acid receptor (RAR) agonists are speculated to be one possible cause for the widely observed frog deformities in North America, although little is known about the specific RAR agonists in aquatic environments. We identified the specific RAR agonists in sewage treatment plants (STPs) and receiving rivers using an RAR yeast two-hybrid bioassay. Water samples were extracted by solid-phase extract cartridges, which were successively eluted by hexane, ethyl acetate, and methanol for bioassay. Among the three fractions, the ethyl acetate fraction showed the highest RAR agonistic activities. The bioassay-derived activity, expressed as all-trans-retinoic acid (all-trans-RA) equivalents (ATRA-EQ) were 10.9 +/- 2.2 and 1.7 +/- 1.0 ng/L in the STP influents and effluents, respectively, while the ATRA-EGs were as high as 7.1 and 8.3 ng/L in the two rivers receiving STP effluents. Following a two-step fractionation using high-performance liquid chromatography and ultra-performance liquid chromatography (UPLC) directed by the bioassay,two bioactive fractions were obtained from Gaobeidian STP influent and all-trans-4-oxo-RA (4.7-10.4 ng/L in influents, <0.2-0.9 ng/L in effluents) and 13-cis-4-oxo-RA (2.3-7.1 ng/L in influents, <0.4-1.1 ng/L in effluents) were identified in these fractions with UPLC-MS/MS. The EC(50) for all-trans-4-oxo-RA or 13-cis-4-oxo-RA relative to that of all-trans-RA in exhibiting RAR alpha agonistic activity was calculated to be 3.87 and 0.46, respectively. |
| WOS记录号 | WOS:000269258000032 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21688]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhen, Huajun,Wu, Xiaoqin,Hu, Jianying,et al. Identification of Retinoic Acid Receptor Agonists in Sewage Treatment Plants[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2009,43(17):6611-6616. |
| APA | Zhen, Huajun.,Wu, Xiaoqin.,Hu, Jianying.,Xiao, Yang.,Yang, Min.,...&Ike, Michihiko.(2009).Identification of Retinoic Acid Receptor Agonists in Sewage Treatment Plants.ENVIRONMENTAL SCIENCE & TECHNOLOGY,43(17),6611-6616. |
| MLA | Zhen, Huajun,et al."Identification of Retinoic Acid Receptor Agonists in Sewage Treatment Plants".ENVIRONMENTAL SCIENCE & TECHNOLOGY 43.17(2009):6611-6616. |
入库方式： OAI收割
来源：生态环境研究中心
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