Intracellular oxidative stress and cadmium ions release induce cytotoxicity of unmodified cadmium sulfide quantum dots
文献类型：期刊论文
| 作者 | Li, K. G. ; Chen, J. T. ; Bai, S. S. ; Wen, X. ; Song, S. Y. ; Yu, Q. ; Li, J. ; Wang, Y. Q. |
| 刊名 | TOXICOLOGY IN VITRO
 |
| 出版日期 | 2009-09 |
| 卷号 | 23期号:6页码:1007-1013 |
| 关键词 | Quantum dots Cadmium ROS GSH Cytotoxicity |
| 中文摘要 | Objective: To fully understand the cytotoxicity of after-degradation QDs, we synthesized CdS QDs and investigated its toxicity mechanism. Methods: Biomimetic method was proposed to synthesize cadmium sulfide (CdS) QDs. Thereafter MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay was conducted to evaluate their cytotoxicity. To investigate the toxicity mechanism, we subsequently conducted intracellular reactive oxygen species (ROS) measurement with DCFH-DA, glutathione (GSH) measurement with DTNB, and cellular cadmium assay using atomic absorption spectrometer. Microsized CdS were simultaneously tested as a comparison. Results: MTT assay results indicated that CdS QDs are more toxic than microsized CdS especially at concentrations below 40 mu g/ml. While microsized CdS did not trigger ROS elevation, CdS QDs increase ROS by 20-30% over control levels. However, they both deplete cellular GSH significantly at the medium concentration of 20 mu g/ml. In the presence of NAC, cells are partially protected from CdS QDs, but not from microsized particles. Additionally, nearly 20% of cadmium was released from CdS nanoparticles within 24 h, which also accounts for QDs' toxicity. Conclusion: Intracellular ROS production, GSH depletion. and cadmium ions (Cd(2+)) release are possible mechanisms for CdS QDs' cytotoxicity. We also suggested that with QD concentration increasing, the principal toxicity mechanism changes from intracellular oxidative stress to Cd(2+) release. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved. |
| WOS记录号 | WOS:000269551500007 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21696]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Li, K. G.,Chen, J. T.,Bai, S. S.,et al. Intracellular oxidative stress and cadmium ions release induce cytotoxicity of unmodified cadmium sulfide quantum dots[J]. TOXICOLOGY IN VITRO,2009,23(6):1007-1013. |
| APA | Li, K. G..,Chen, J. T..,Bai, S. S..,Wen, X..,Song, S. Y..,...&Wang, Y. Q..(2009).Intracellular oxidative stress and cadmium ions release induce cytotoxicity of unmodified cadmium sulfide quantum dots.TOXICOLOGY IN VITRO,23(6),1007-1013. |
| MLA | Li, K. G.,et al."Intracellular oxidative stress and cadmium ions release induce cytotoxicity of unmodified cadmium sulfide quantum dots".TOXICOLOGY IN VITRO 23.6(2009):1007-1013. |
入库方式： OAI收割
来源：生态环境研究中心
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