A two-hybrid yeast assay to quantify the effects of xenobiotics on retinoid X receptor-mediated gene expression
文献类型：期刊论文
| 作者 | Li, Jian ; Ma, Mei ; Wang, Zijian |
| 刊名 | TOXICOLOGY LETTERS
 |
| 出版日期 | 2008-02-15 |
| 卷号 | 176期号:3页码:198-206 |
| 关键词 | in vitro bioassay retinoid X receptor disruption retinoic acid yeast two-hybrid assay |
| 英文摘要 | The aim of this study is to investigate the chemical retinoic acid (RA) disruption at the level of retinoid X receptor (RXR) functioning. This assay makes use of recombined human RXR gene and reporter gene yeast, which specifically expresses P-galactosidase when incubated with exogenous 9-cis retinoic acid (9-cis RA). Agonistic and antagonistic actions of chemicals including a series of phenols, phthalates, organochlorine pesticides (OCPs) were tested in the absence and presence of 5 x 10(-6) mol/L 9-cis RA, at which maximal P-galactosidase activity could be induced. The results obtained reveal that some chemicals, e.g., 2-t-butylphenol, 2-isopropylphenol, 2,4-dichlorophenol (2,4-DCP), 3,4-dichlorophenol (3,4-DCP), 4-tert-octylphenol (4-t-OP) and hexachlorobenzene (HCB), are RXR agonists. Especially, bisphenol A (BPA) showed high induction activity to RXR when tested with metabolization. The 20% relative inhibitory concentration (RIC20) values of r-hexachlorocyclohexane (HCH), p,p'-dichlorodiphenyltrichloroethane (p,p'-DDT) and 2,4-DCP with metabolization were lower than 1 x 10(-6) mol/L. These results suggest that BPA, HCH, pp'-DDT and 2,4-DCP are chemicals that pose a threat to hRXR functioning. Altogether the results of the present study show that p the newly developed, yeast two-hybrid assay can be used as a valuable tool for identification and quantification of compounds active in disturbing retinoid homeostasis at the level of RXR. (c) 2007 Elsevier Ireland Ltd. All rights reserved. |
| WOS记录号 | WOS:000254076700004 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/22202]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Li, Jian,Ma, Mei,Wang, Zijian. A two-hybrid yeast assay to quantify the effects of xenobiotics on retinoid X receptor-mediated gene expression[J]. TOXICOLOGY LETTERS,2008,176(3):198-206. |
| APA | Li, Jian,Ma, Mei,&Wang, Zijian.(2008).A two-hybrid yeast assay to quantify the effects of xenobiotics on retinoid X receptor-mediated gene expression.TOXICOLOGY LETTERS,176(3),198-206. |
| MLA | Li, Jian,et al."A two-hybrid yeast assay to quantify the effects of xenobiotics on retinoid X receptor-mediated gene expression".TOXICOLOGY LETTERS 176.3(2008):198-206. |
入库方式： OAI收割
来源：生态环境研究中心
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