Influences of 4-nonylphenol on doublesex- and mab-3-related transcription factor I gene expression and vitellogenin mRNA induction of adult rare minnow (Gobiocypris rarus)
文献类型：期刊论文
| 作者 | Zhang, Xiaoyan; Zha, Jinmiao; Wang, Zijian |
| 刊名 | ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
 |
| 出版日期 | 2008-01 |
| 卷号 | 27期号:1页码:196-205 |
| 关键词 | doublesex/mab-3 domain vitellogenin rare minnow (Gobiocypris rarus) 4-nonylphenol |
| 英文摘要 | The mab-3 of Caenorhabditis elegans and doublesex of Drosophila are two sex-determining genes that are found to have only a common DNA binding motif called a doublesex and mab-3 (DM) domain among phyla. The DM domain regulates some aspects of sexual development such as yolk protein synthesis. In the present study, by using DM degenerate primers' 13 sequences of doublesex and mab-3-related transcription factor (dmrtI), genes were cloned from genomic DNA of rare minnow (Gobiocypris rarus) and expression of dmrtI was detected only in testis of adult males. In addition, partial sequences of vitellogenin (vtg) and beta-actin genes as comparative expression of genes were cloned from the female liver by degenerate primers based on conserved regions. The vtg mRNA in male liver was induced significantly in concentrations higher than 2 mu g/L treatments, although adult rare minnow were exposed to nominal concentrations of 2. 10, 50, 250, and 1,250 mu g/L 4-nonylphenol (4-NP) for 21 d. The dmrtl mRNA level was significantly downregulated in a dose-dependent manner in all exposure groups of males. Moreover, other toxic endpoints, including growth, gonadosomatic index, hepatosomatic index, and histopathology, were determined. However, significant effects were observed only at higher exposure concentrations. Therefore, sex-specific genes such as dmrtI can be used as a more sensitive biomarker for 4-NP and for discovering molecular mechanisms underlying toxic response. |
| WOS记录号 | WOS:000251759500029 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/22252]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang, Xiaoyan,Zha, Jinmiao,Wang, Zijian. Influences of 4-nonylphenol on doublesex- and mab-3-related transcription factor I gene expression and vitellogenin mRNA induction of adult rare minnow (Gobiocypris rarus)[J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,2008,27(1):196-205. |
| APA | Zhang, Xiaoyan,Zha, Jinmiao,&Wang, Zijian.(2008).Influences of 4-nonylphenol on doublesex- and mab-3-related transcription factor I gene expression and vitellogenin mRNA induction of adult rare minnow (Gobiocypris rarus).ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,27(1),196-205. |
| MLA | Zhang, Xiaoyan,et al."Influences of 4-nonylphenol on doublesex- and mab-3-related transcription factor I gene expression and vitellogenin mRNA induction of adult rare minnow (Gobiocypris rarus)".ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY 27.1(2008):196-205. |
入库方式： OAI收割
来源：生态环境研究中心
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