Fluorescence and SEM correlative microscopy for nanomanipulation of subcellular structures
文献类型：期刊论文
| 作者 | Gong, Zheng1; Chen, Brandon K.1; Liu, Jun1; Zhou, Chao2 ; Anchel, Dave3; Li, Xiao1; Ge, Ji1; Bazett-Jones, David P.3; Sun, Yu1
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| 刊名 | LIGHT-SCIENCE & APPLICATIONS
 |
| 出版日期 | 2014-11-01 |
| 卷号 | 3 |
| 关键词 | correlative microscopy fluorescence image correlation nanomanipulation SEM subcellular structures |
| 英文摘要 | Nanomanipulation under scanning electron microscopy (SEM) enables direct interactions of a tool with a sample. We recently developed a nanomanipulation technique for the extraction and identification of DNA contained within sub-nuclear locations of a single cell nucleus. In nanomanipulation of sub-cellular structures, a key step is to identify targets of interest through correlating fluorescence and SEM images. The DNA extraction task must be conducted with low accelerating voltages resulting in low imaging resolutions. This is imposed by the necessity of preserving the biochemical integrity of the sample. Such poor imaging conditions make the identification of nanometer-sized fiducial marks difficult. This paper presents an affine scale-invariant feature transform (ASIFT) based method for correlating SEM images and fluorescence microscopy images. The performance of the image correlation approach under different noise levels and imaging magnifications was quantitatively evaluated. The optimal mean absolute error (MAE) of correlation results is 68 +/- 34 nm under standard conditions. Compared with manual correlation by skilled operators, the automated correlation approach demonstrates a speed that is higher by an order of magnitude. With the SEM-fluorescence image correlation approach, targeted DNA was successfully extracted via nanomanipulation under SEM conditions. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Optics |
| 研究领域[WOS] | Optics |
| 关键词[WOS] | ELECTRON-MICROSCOPY ; NUCLEAR-BODIES ; DNA ; GENOME ; ORGANIZATION ; DISEASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000346707000002 |
| 源URL | [http://ir.ia.ac.cn/handle/173211/3504]  |
| 专题 | 自动化研究所_复杂系统管理与控制国家重点实验室_先进机器人控制团队 |
| 作者单位 | 1.Univ Toronto, Adv Micro & Nanosyst Lab, Toronto, ON M5S 3G8, Canada 2.Chinese Acad Sci, Inst Automat, State Key Lab Intelligent Control & Management Co, Beijing 100190, Peoples R China 3.Hosp Sick Children, Genet & Genome Biol Program, Toronto, ON M5G 1X8, Canada |
| 推荐引用方式 GB/T 7714 | Gong, Zheng,Chen, Brandon K.,Liu, Jun,et al. Fluorescence and SEM correlative microscopy for nanomanipulation of subcellular structures[J]. LIGHT-SCIENCE & APPLICATIONS,2014,3. |
| APA | Gong, Zheng.,Chen, Brandon K..,Liu, Jun.,Zhou, Chao.,Anchel, Dave.,...&Sun, Yu.(2014).Fluorescence and SEM correlative microscopy for nanomanipulation of subcellular structures.LIGHT-SCIENCE & APPLICATIONS,3. |
| MLA | Gong, Zheng,et al."Fluorescence and SEM correlative microscopy for nanomanipulation of subcellular structures".LIGHT-SCIENCE & APPLICATIONS 3(2014). |
入库方式： OAI收割
来源：自动化研究所
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