SM5-1-Conjugated PLA nanoparticles loaded with 5-fluorouracil for targeted hepatocellular carcinoma imaging and therapy
文献类型：期刊论文
| 作者 | Ma, Xibo1 ; Cheng, Zhen2; Jin, Yushen3; Liang, Xiaolong4; Yang, Xin1; Dai, Zhifei4; Tian, Jie1
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| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2014-03-01 |
| 卷号 | 35期号:9页码:2878-2889 |
| 关键词 | Nanoparticles Bioluminescence imaging (BLI) Bioluminescent tomography (BLT) Targeted imaging and therapy |
| 英文摘要 | SM5-1 is a humanized mouse antibody which has a high binding specificity for a membrane protein of about 230 kDa overexpressed in hepatocellular carcinoma (HCC), melanoma and breast cancer. In this study, SM5-1-conjugated poly D, I. (lactide-coglycolide) (PLA) PLA containing Cy7 (PLA-Cy7-SM5-1) was prepared to study the targeting specificity of the bioconjugate to HCC-LM3-fLuc cell. Then, SM5-1-conjugated PLA containing 5-fluorouracil (5-FU) (PLA-5FU-SM5-1) and PLA containing 5-FU (PLA-5FU) were prepared for treatment of subcutaneous HCC-LM3-fLuc tumor mice. The results showed that PLA-5FU-SM5-1, PLA-5FU and 5-FU induced a 45.07%, 23.56% and 19.05% tumor growth inhibition rate, respectively, on day 31 post-treatment as determined by bioluminescent intensity. In addition, in order to evaluate the antitumor efficacy of PLA-5FU-SM5-1, HCC-LM3-fLuc cells were injected into the liver to establish the experimental orthotopic liver tumor models. The experiments showed that PLA-5FU-SM5-1, PLA-5FU and 5-FU induced a 53.24%, 31.00%, and 18.11% tumor growth inhibition rate, respectively, on day 31 post-treatment determined by the bioluminescent intensity of the abdomen in tumor-bearing mice. Furthermore, we have calculated the three-dimensional location of the liver cancer in mice using a multilevel adaptive finite element algorithm based on bioluminescent intensity decay calibration. The reconstruction results demonstrated that PLA-5FU-SM5-1 inhibited the tumor rapid progression, which were consistent with the results of subcutaneous tumor mice experiments and in vitro cell experiment results. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Biomedical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Engineering ; Materials Science |
| 关键词[WOS] | PROTEIN-PROTEIN INTERACTIONS ; POSITRON-EMISSION-TOMOGRAPHY ; GROWTH-FACTOR RECEPTOR ; BIOLUMINESCENCE TOMOGRAPHY ; IN-VIVO ; BREAST-CANCER ; MONOCLONAL-ANTIBODY ; 1ST-LINE TREATMENT ; COLORECTAL-CANCER ; TUMOR-METASTASIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000332188900031 |
| 源URL | [http://ir.ia.ac.cn/handle/173211/4033]  |
| 专题 | 自动化研究所_中国科学院分子影像重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Automat, Key Lab Mol Imaging, Beijing 100190, Peoples R China 2.Stanford Univ, Dept Radiol, MIPS, BioX Program, Stanford, CA 94305 USA 3.Harbin Inst Technol, Sch Life Sci & Technol, Nanomed & Biosensor Lab, Harbin 150080, Peoples R China 4.Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Xibo,Cheng, Zhen,Jin, Yushen,et al. SM5-1-Conjugated PLA nanoparticles loaded with 5-fluorouracil for targeted hepatocellular carcinoma imaging and therapy[J]. BIOMATERIALS,2014,35(9):2878-2889. |
| APA | Ma, Xibo.,Cheng, Zhen.,Jin, Yushen.,Liang, Xiaolong.,Yang, Xin.,...&Tian, Jie.(2014).SM5-1-Conjugated PLA nanoparticles loaded with 5-fluorouracil for targeted hepatocellular carcinoma imaging and therapy.BIOMATERIALS,35(9),2878-2889. |
| MLA | Ma, Xibo,et al."SM5-1-Conjugated PLA nanoparticles loaded with 5-fluorouracil for targeted hepatocellular carcinoma imaging and therapy".BIOMATERIALS 35.9(2014):2878-2889. |
入库方式： OAI收割
来源：自动化研究所
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