中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
ApoG2 as the most potent gossypol derivatives inhibits cell growth and induces apoptosis on gastric cancer cells

文献类型:期刊论文

作者Xin, J.1; Zhan, Y. H.1; Xia, L. M.3,4; Zhu, H. W.3,4,5; Nie, Y. Z.3,4; Liang, J. M.1; Tian, J.1,2; Tian, Jie
刊名BIOMEDICINE & PHARMACOTHERAPY
出版日期2013-02-01
卷号67期号:1页码:88-95
关键词ApoG2 Small-molecule inhibitor Gastric cancer
英文摘要Gastric cancer is one of the most common types of malignancies and proteins from the Bcl-2 family are highly expressed in human gastric cancer. Apogossypolone (ApoG2), the most potent gossypol derivative, has been defined as a novel small-molecule inhibitor of anti-apoptotic Bcl-2 family proteins. However, whether or not it can inhibit the growth and proliferation of gastric cancer cell lines has not been demonstrated to date. Here, we assessed the effects of anti-growth of ApoG2 on gastric cancer cell lines in vitro and explored the possible molecular mechanisms of ApoG2. Using the MTT assay and flow cytometry, we found that ApoG2 has the significant anti-growth effect on MKN28, MKN45 and AGS cell lines in a time-and dose-dependent manner. Compared to (-)-gossypol, MTT assay and flow cytometry results showed that anti-growth effect of ApoG2 is inferior, but the colony formation ability of ApoG2 is superior. Furthermore, western blot results revealed that ApoG2 inhibits the growth and proliferation of gastric cancer cells by down-regulating of Bcl-2 protein expression, up-regulating of Bax and activating of Caspase-3. Taken together, albeit the ApoG2 inferior to (-)-gossypol in many ways on gastric cancer in vitro, our results suggest that ApoG2 could effectively inhibit the growth and proliferation of gastric cancer cell lines through the mitochondrial pathway of apoptosis. (C) 2012 Elsevier Masson SAS. All rights reserved.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]BCL-2 FAMILY PROTEINS ; PROSTATE-CANCER ; BREAST-CANCER ; CYCLE ARREST ; U937 CELLS ; IN-VITRO ; APOGOSSYPOLONE ; THERAPY ; CARCINOMA ; PATHWAYS
收录类别SCI
语种英语
WOS记录号WOS:000317096300014
源URL[http://ir.ia.ac.cn/handle/173211/4039]  
专题自动化研究所_中国科学院分子影像重点实验室
通讯作者Tian, Jie
作者单位1.Xidian Univ, Sch Life Sci & Technol, Xian 710071, Shaanxi, Peoples R China
2.Chinese Acad Sci, Inst Automat, Beijing 100190, Peoples R China
3.Fourth Mil Med Univ, Xijing Hosp, Dept Gastroenterol, Xian 710032, Shaanxi, Peoples R China
4.Fourth Mil Med Univ, Xijing Hosp, State Key Lab Canc Biol, Xian 710032, Shaanxi, Peoples R China
5.Guangzhou Mil Command, Guangzhou Gen Hosp, Dept Gastroenterol, Guangzhou 510010, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Xin, J.,Zhan, Y. H.,Xia, L. M.,et al. ApoG2 as the most potent gossypol derivatives inhibits cell growth and induces apoptosis on gastric cancer cells[J]. BIOMEDICINE & PHARMACOTHERAPY,2013,67(1):88-95.
APA Xin, J..,Zhan, Y. H..,Xia, L. M..,Zhu, H. W..,Nie, Y. Z..,...&Tian, Jie.(2013).ApoG2 as the most potent gossypol derivatives inhibits cell growth and induces apoptosis on gastric cancer cells.BIOMEDICINE & PHARMACOTHERAPY,67(1),88-95.
MLA Xin, J.,et al."ApoG2 as the most potent gossypol derivatives inhibits cell growth and induces apoptosis on gastric cancer cells".BIOMEDICINE & PHARMACOTHERAPY 67.1(2013):88-95.

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来源:自动化研究所

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