In Vitro and In Vivo Evaluation of a Humanized Anti-APRIL Antibody
文献类型：期刊论文
| 作者 | Gao, Q.1; Li, Q.2; Xue, Z.3; Wu, P.3 ; Yang, X.3
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| 刊名 | CURRENT MOLECULAR MEDICINE
 |
| 出版日期 | 2013-03-01 |
| 卷号 | 13期号:3页码:464-465 |
| 关键词 | A proliferation-inducing ligand APRIL tumor necrosis factor (TNF) tumorigenesis |
| 英文摘要 | A proliferation-inducing ligand (APRIL) is overexpressed in many cancers such as colorectal, gastric and liver. Silence APRIL gene or neutralize its expression may serve as therapeutic strategies to manage those cancers. Current phase I clinical trial shows that heavily pretreated patients were well tolerated high dose intravenous infusion. Herein, we report a humanized antibody (Ab) that neutralizes APRIL protein and inhibits cancer cell proliferation both in vitro and in vivo. The Ab was generated through conjugating foreign immunodominant T-helper cell (Th) epitopes to the N- or C-terminal of four soluble APRIL mutants. In vitro anti-proliferation effects were evaluated in both Raji and Jurkat cell lines. In vivo inhibition of tumor growth was determined in a tumor xenograft animal model with green fluorescent protein (GFP) positive Raji cell line. In vitro data demonstrated that this Ab significantly inhibited both Raji and Jurkat cell proliferation in a dose-dependent manner. The molecular imaging data demonstrated in vivo tumor growth delay when animals were treated with Ab. In conclusion, we have developed a humanized anti-APRIL Ab, which is effective in inhibiting tumor cell proliferations both in vitro and vivo. The humanized Ab has potential to reduce the immunoresponse in the clinical situation. This Ab also can be used in other APRIL mediated diseases such as Sjogren syndrome, multiple sclerosis and systemic lupus erythematosus. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Medicine, Research & Experimental |
| 研究领域[WOS] | Research & Experimental Medicine |
| 关键词[WOS] | CHRONIC LYMPHOCYTIC-LEUKEMIA ; BAFF ; EXPRESSION ; SURVIVAL ; BLYS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000317116100016 |
| 源URL | [http://ir.ia.ac.cn/handle/173211/4049]  |
| 专题 | 自动化研究所_中国科学院分子影像重点实验室 |
| 作者单位 | 1.Acad Mil Med Sci, Lab Anim Ctr, Beijing 100850, Peoples R China 2.Chongqing Sci & Technol Commiss, Intellectual Property Bur, Chongqing, Peoples R China 3.Chinese Acad Sci, Inst Automat, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Q.,Li, Q.,Xue, Z.,et al. In Vitro and In Vivo Evaluation of a Humanized Anti-APRIL Antibody[J]. CURRENT MOLECULAR MEDICINE,2013,13(3):464-465. |
| APA | Gao, Q.,Li, Q.,Xue, Z.,Wu, P.,&Yang, X..(2013).In Vitro and In Vivo Evaluation of a Humanized Anti-APRIL Antibody.CURRENT MOLECULAR MEDICINE,13(3),464-465. |
| MLA | Gao, Q.,et al."In Vitro and In Vivo Evaluation of a Humanized Anti-APRIL Antibody".CURRENT MOLECULAR MEDICINE 13.3(2013):464-465. |
入库方式： OAI收割
来源：自动化研究所
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