Cerenkov Luminescence Tomography of Aminopeptidase N (APN/CD13) Expression in Mice Bearing HT1080 Tumors
文献类型：期刊论文
| 作者 | Hu, Zhenhua ; Yang, Weidong; Ma, Xiaowei; Ma, Wenhui; Qu, Xiaochao; Liang, Jimin1; Wang, Jing; Tian, Jie
|
| 刊名 | MOLECULAR IMAGING
 |
| 出版日期 | 2013-05-01 |
| 卷号 | 12期号:3页码:- |
| 英文摘要 | In vivo imaging of aminopeptidase N (APN/CD13) expression is crucial for the early detection of cancer. This study attempted to show that APN/CD13 expression can be imaged and quantified with novel Cerenkov luminescence tomography (CLT). (NaI)-I-131 with various activities was placed at different depths in a tissue-mimicking phantom, and various porcine tissues and luminescent images were acquired. The binding of I-131-NGR with human fibrosarcoma HT1080 and human colon cancer HT-29 cells was detected with Cerenkov luminescence imaging (CLI). Nude mice bearing HT-1080 tumors were imaged after injection with I-131-NGR using both planar and tomographic CLI methods. The penetration depth increased with ascending activity of (NaI)-I-131. There was a robust linear correlation between the optical signal intensity and the HT1080 cell numbers (r(2) = .9691), as well as the activity (r(2) = .9860). The three-dimensional visualization CLT results clearly showed that I-131-NGR uptake in tumor tissues represented a high expression of the APN/CD13 receptor. CLT also allowed quantifying I-131-NGR uptake in tumor tissues showing an average activity of 0.1388 +/- 4.6788E-6 MBq in tumor tissues. Our study indicated that I-131-NGR combined with CLT allowed us to image and quantify tumor-associated APN/CD13 expression noninvasively. The promising CLT technique could be potentially used for sensitively evaluating tumor angiogenesis in vivo. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods ; Radiology, Nuclear Medicine & Medical Imaging |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Radiology, Nuclear Medicine & Medical Imaging |
| 关键词[WOS] | BIOLUMINESCENCE TOMOGRAPHY ; OPTICAL-PROPERTIES ; MODEL ; RECONSTRUCTION ; ANGIOGENESIS ; RADIOTRACERS ; RADIATION ; MEDIA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000336723700006 |
| 源URL | [http://ir.ia.ac.cn/handle/173211/4087]  |
| 专题 | 自动化研究所_中国科学院分子影像重点实验室 |
| 作者单位 | 1.Xidian Univ, Sch Life Sci & Technol, Xian 710071, Peoples R China 2.Fourth Mil Med Univ, Xijing Hosp, Dept Nucl Med, Xian 710032, Peoples R China 3.Chinese Acad Sci, Inst Automat, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Zhenhua,Yang, Weidong,Ma, Xiaowei,et al. Cerenkov Luminescence Tomography of Aminopeptidase N (APN/CD13) Expression in Mice Bearing HT1080 Tumors[J]. MOLECULAR IMAGING,2013,12(3):-. |
| APA | Hu, Zhenhua.,Yang, Weidong.,Ma, Xiaowei.,Ma, Wenhui.,Qu, Xiaochao.,...&Tian, Jie.(2013).Cerenkov Luminescence Tomography of Aminopeptidase N (APN/CD13) Expression in Mice Bearing HT1080 Tumors.MOLECULAR IMAGING,12(3),-. |
| MLA | Hu, Zhenhua,et al."Cerenkov Luminescence Tomography of Aminopeptidase N (APN/CD13) Expression in Mice Bearing HT1080 Tumors".MOLECULAR IMAGING 12.3(2013):-. |
入库方式： OAI收割
来源：自动化研究所
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