In-vivo Optical Tomography of Small Scattering Specimens: time-lapse 3D imaging of the head eversion process in Drosophila melanogaster
文献类型：期刊论文
| 作者 | Arranz, Alicia1; Dong, Di2; Zhu, Shouping3; Savakis, Charalambos4; Tian, Jie2,3; Ripoll, Jorge5,6; Di Dong ; Jie Tian
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| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2014-12-04 |
| 卷号 | 4页码:- |
| 英文摘要 | Even though in vivo imaging approaches have witnessed several new and important developments, specimens that exhibit high light scattering properties such as Drosophila melanogaster pupae are still not easily accessible with current optical imaging techniques, obtaining images only from subsurface features. This means that in order to obtain 3D volumetric information these specimens need to be studied either after fixation and a chemical clearing process, through an imaging window - thus perturbing physiological development -, or during early stages of development when the scattering contribution is negligible. In this paper we showcase how Optical Projection Tomography may be used to obtain volumetric images of the head eversion process in vivo in Drosophila melanogaster pupae, both in control and headless mutant specimens. Additionally, we demonstrate the use of Helical Optical Projection Tomography (hOPT) as a tool for high throughput 4D-imaging of several specimens simultaneously. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | PLANE ILLUMINATION MICROSCOPY ; PROJECTION TOMOGRAPHY ; GENE-EXPRESSION ; BRAIN ; ZEBRAFISH ; ANATOMY ; TOOL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000346268300005 |
| 源URL | [http://ir.ia.ac.cn/handle/173211/4110]  |
| 专题 | 自动化研究所_中国科学院分子影像重点实验室 |
| 作者单位 | 1.Swiss Fed Inst Technol, Inst Biomed Engn, CH-8093 Zurich, Switzerland 2.Chinese Acad Sci, Inst Automat, Beijing 100190, Peoples R China 3.Xidian Univ, Sch Life Sci & Technol, Xian 710071, Shaanxi, Peoples R China 4.BSRC Alexander Fleming, Varkiza 16602, Greece 5.Univ Carlos III Madrid, Dept Bioengn & Aerosp Engn, Madrid 28911, Spain 6.Sanitaria Hosp Gregorio Maranon, Inst Invest, Expt Med & Surg Unit, Madrid 28007, Spain |
| 推荐引用方式 GB/T 7714 | Arranz, Alicia,Dong, Di,Zhu, Shouping,et al. In-vivo Optical Tomography of Small Scattering Specimens: time-lapse 3D imaging of the head eversion process in Drosophila melanogaster[J]. SCIENTIFIC REPORTS,2014,4:-. |
| APA | Arranz, Alicia.,Dong, Di.,Zhu, Shouping.,Savakis, Charalambos.,Tian, Jie.,...&Jie Tian.(2014).In-vivo Optical Tomography of Small Scattering Specimens: time-lapse 3D imaging of the head eversion process in Drosophila melanogaster.SCIENTIFIC REPORTS,4,-. |
| MLA | Arranz, Alicia,et al."In-vivo Optical Tomography of Small Scattering Specimens: time-lapse 3D imaging of the head eversion process in Drosophila melanogaster".SCIENTIFIC REPORTS 4(2014):-. |
入库方式： OAI收割
来源：自动化研究所
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