Plant regeneration of an endangered medicinal plant Hydrastis canadensis L.
文献类型：期刊论文
| 作者 | He, Shan-Shan; Liu, Chun-Zhao; Saxena, Praveen K. |
| 刊名 | SCIENTIA HORTICULTURAE
 |
| 出版日期 | 2007-06-05 |
| 卷号 | 113期号:1页码:82-86 |
| 关键词 | bioreactor culture germplasm conservation goldenseal hydrastis canadensis L. thidiazuron |
| ISSN号 | 0304-4238 |
| 英文摘要 | Goldenseal (Hydrastis canadensis L.) is an endangered medicinal plant used to treat sore eyes and mouths, cold and flu and also as a dye. The objective of this study was to develop an efficient in vitro propagation protocol for goldenseal. Significantly more shoots (26 shoots per leaf explants) were induced on a medium containing 2.5 mu M thidiazuron (TDZ) and 5.0 mu M1-naphthaleneacetic acid (NAA) than any other treatment. Sub-culturing regenerated shoots on a medium with 5.0 mu M 6-benzylaminopurine (BA) induced the maximum rate of shoot multiplication. Growth of the regenerated shoots in a temporary immersion bioreactor resulted in significant increases in biomass, shoot height and shoot multiplication. The regenerated shoots from the temporary immersion bioreactor formed roots when transferred onto a medium with 1.0-2.0 RM indole-3-butyric acid (IBA). Regenerated whole plantlets were acclimatized and maintained in standard greenhouse conditions for further growth. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of this rare, medicinally important species. (C) 2007 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Horticulture |
| 研究领域[WOS] | Agriculture |
| 关键词[WOS] | ARTEMISIA-JUDAICA L ; MICROPROPAGATION ; GOLDENSEAL ; CULTURE ; BIOREACTORS ; CLUSTERS ; GROWTH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000247261700014 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/19214]  |
| 专题 | 过程工程研究所_多相复杂系统国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Shan-Shan,Liu, Chun-Zhao,Saxena, Praveen K.. Plant regeneration of an endangered medicinal plant Hydrastis canadensis L.[J]. SCIENTIA HORTICULTURAE,2007,113(1):82-86. |
| APA | He, Shan-Shan,Liu, Chun-Zhao,&Saxena, Praveen K..(2007).Plant regeneration of an endangered medicinal plant Hydrastis canadensis L..SCIENTIA HORTICULTURAE,113(1),82-86. |
| MLA | He, Shan-Shan,et al."Plant regeneration of an endangered medicinal plant Hydrastis canadensis L.".SCIENTIA HORTICULTURAE 113.1(2007):82-86. |
入库方式： OAI收割
来源：过程工程研究所
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