Effect of IPTG amount on apo- and holo- forms of glycerophosphate oxidase expressed in Escherichia coli
文献类型：期刊论文
| 作者 | Zheng, Chunyang1; Zhao, Zhanyong2; Li, Yan3; Wang, Lin1,2; Su, Zhiguo3 |
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2011-02-01 |
| 卷号 | 75期号:2页码:133-137 |
| 关键词 | Apo-GPO Holo GPO FAD Cofactor Specific activity |
| ISSN号 | 1046-5928 |
| 英文摘要 | Escherichia coli has proved to be a successful host for the expression of many heterologous proteins and much efforts have been made toward improving recombinant protein expression including the usage of strong promoters and co-expression with chaperones But little attention was paid on the relation between expression level and function of the target protein Glycerophosphate oxidase (GPO) is a protein with FAD cofactor (without free cysteine and disulfide bonds) It was observed that the specific activity of GPO dramatically decreased with the increase of inducer IPTG In addition the stability of it decreased correspondingly The structural difference of samples expressed under varying IPTG was investigated using size-exclusion and reverse-phase high performance liquid chromatography together with CD spectrum It was found that the conformation of peptide and organization of subunits were not affected The loss of specific activity and stability were correlated to Incomplete attachment of FAD onto GPO These results revealed that synthesis speed should be controlled either by reduction of IPTG amount or using weak promoters in the production of GPO (C) 2010 Elsevier Inc All rights reserved |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | AMINO-ACID OXIDASE ; DIMETHYLGLYCINE DEHYDROGENASE ; 6-HYDROXY-D-NICOTINE OXIDASE ; BACILLUS-SUBTILIS ; COFACTOR BINDING ; GLYCINE OXIDASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000285126500003 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/19228]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Ctr Struct & Mol Biol, Inst Biophys, Beijing 100101, Peoples R China 2.Biotrand Inc, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Chunyang,Zhao, Zhanyong,Li, Yan,et al. Effect of IPTG amount on apo- and holo- forms of glycerophosphate oxidase expressed in Escherichia coli[J]. PROTEIN EXPRESSION AND PURIFICATION,2011,75(2):133-137. |
| APA | Zheng, Chunyang,Zhao, Zhanyong,Li, Yan,Wang, Lin,&Su, Zhiguo.(2011).Effect of IPTG amount on apo- and holo- forms of glycerophosphate oxidase expressed in Escherichia coli.PROTEIN EXPRESSION AND PURIFICATION,75(2),133-137. |
| MLA | Zheng, Chunyang,et al."Effect of IPTG amount on apo- and holo- forms of glycerophosphate oxidase expressed in Escherichia coli".PROTEIN EXPRESSION AND PURIFICATION 75.2(2011):133-137. |
入库方式： OAI收割
来源：过程工程研究所
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