A hydrophobic interaction chromatography strategy for purification of inactivated foot-and-mouth disease virus
文献类型：期刊论文
| 作者 | Li, Hao1,2; Yang, Yanli1,2; Zhang, Yan1; Zhang, Songping1; Zhao, Qizu3; Zhu, Yuanyuan3; Zou, Xingqi3; Yu, Mengran1,2; Ma, Guanghui1; Su, Zhiguo1 |
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2015-09-01 |
| 卷号 | 113期号:SEP页码:23-29 |
| 关键词 | Foot-and-mouth disease virus Hydrophobic interaction chromatography Purification Downstream processing |
| ISSN号 | 1046-5928 |
| 英文摘要 | A purification scheme based on hydrophobic interaction chromatography was developed to separate inactivated foot-and-mouth disease virus (FMDV) from crude supernatant. About 92% recovery and 8.8-fold purification were achieved on Butyl Sepharose 4FF. Further purification on Superdex 200 resulted in another 29-fold purification, with 92% recovery. The columns were coupled through an intermediate ultrafiltration unit to concentrate the virus. The entire process was completed in about 3.5 h, with 75% final FMDV recovery, and 247-fold purification. The final product had purity above 98%, with over 99.5% of host cell DNA removed. High-performance size exclusion chromatography (HPSEC), Western blot, dynamic light scattering (DLS), and transmission electron microscopy (TEM) indicated that the purified virus contained the required antigen, and was structurally intact with a spherical shape and a particle size of 28 nm. (C) 2015 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | SIZE-EXCLUSION CHROMATOGRAPHY ; ION-EXCHANGE CHROMATOGRAPHY ; B SURFACE-ANTIGEN ; HANSENULA-POLYMORPHA ; PARTICLES ; VACCINES ; QUANTIFICATION ; CAPSIDS ; FMDV ; CELL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000356113300004 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/19297]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.China Inst Vet Drug Control, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Hao,Yang, Yanli,Zhang, Yan,et al. A hydrophobic interaction chromatography strategy for purification of inactivated foot-and-mouth disease virus[J]. PROTEIN EXPRESSION AND PURIFICATION,2015,113(SEP):23-29. |
| APA | Li, Hao.,Yang, Yanli.,Zhang, Yan.,Zhang, Songping.,Zhao, Qizu.,...&Su, Zhiguo.(2015).A hydrophobic interaction chromatography strategy for purification of inactivated foot-and-mouth disease virus.PROTEIN EXPRESSION AND PURIFICATION,113(SEP),23-29. |
| MLA | Li, Hao,et al."A hydrophobic interaction chromatography strategy for purification of inactivated foot-and-mouth disease virus".PROTEIN EXPRESSION AND PURIFICATION 113.SEP(2015):23-29. |
入库方式： OAI收割
来源：过程工程研究所
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