热门
Characterization of the topology and functional domains of RKTG
文献类型：期刊论文
| 作者 | Luo, Xiaolin; Feng, Lin; Jiang, Xiaomeng; Xiao, Fei; Wang, Zhenzhen（王甄真）; Feng, Gen-Sheng; Chen, Yan（陈雁）
|
| 刊名 | BIOCHEMICAL JOURNAL
 |
| 出版日期 | 2008 |
| 卷号 | 414期号:1页码:399-406 |
| 关键词 | Golgi apparatus progesterone and adipoQ receptor (PAQR) Raf-1 RKTG |
| 通讯作者 | Chen, Y (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Blol Sci, Inst Nutrit Sci,Key Lab Nutr & Metab, 294 Taiyuan Rd, Shanghai 200031, Peoples R China. |
| 英文摘要 | RKTG (Raf kinase trapping to Golgi) is exclusively localized at the Golgi apparatus and functions as a spatial regulator of Raf-1 kinase by sequestrating Raf-1 to the Golgi. Based on the structural similarity with adiponectin receptors, RKTG was predicted to be a seven-transmembrane protein with a cytosolic N-terminus, distinct from classical GPCRs (G-protein-coupled receptors). We analysed in detail the topology and functional domains of RKTG in this study. We determined that the N-terminus of RKTG is localized on the cytosolic side. Two short stretches of amino acid sequences at the membrane proximal to the N- and C-termini (amino acids 61-71 and 299-303 respectively) were indispensable for Golgi localization of RKTG, but were not required for the interaction with Raf-1. The three loops facing the cytosol between the transmembrane domains had different roles in Golgi localization and Raf-1 interaction. While the first cytosolic loop was only important for Golgi localization, the third cytosolic loop was necessary for both Golgi localization and Raf-1 sequestration. Taken together, these findings Suggest that RKTG is a type III membrane protein with its N-terminus facing the cytosol and multiple sequences are responsible for its localization at the Golgi apparatus and Raf-1 interaction. As RKTG is the first discovered Golgi protein with seven transmembrane domains, the knowledge derived from this study Would not only provide structural information about the protein, but also pave the way for future characterization of the Unique functions of RKTG in the regulation of cell signalling. |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | MEMBRANE PROGESTIN RECEPTOR ; TRANS-GOLGI NETWORK ; MOLECULAR CHARACTERIZATION ; ADIPONECTIN RECEPTORS ; CYTOPLASMIC DOMAIN ; RAT HEPATOCYTES ; BINDING PROTEIN ; COMPLEX ; IDENTIFICATION ; LOCALIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000259512500009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/103]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_信号转导与营养相关疾病研究组 |
| 推荐引用方式 GB/T 7714 | Luo, Xiaolin,Feng, Lin,Jiang, Xiaomeng,et al. Characterization of the topology and functional domains of RKTG[J]. BIOCHEMICAL JOURNAL,2008,414(1):399-406. |
| APA | Luo, Xiaolin.,Feng, Lin.,Jiang, Xiaomeng.,Xiao, Fei.,Wang, Zhenzhen.,...&Chen, Yan.(2008).Characterization of the topology and functional domains of RKTG.BIOCHEMICAL JOURNAL,414(1),399-406. |
| MLA | Luo, Xiaolin,et al."Characterization of the topology and functional domains of RKTG".BIOCHEMICAL JOURNAL 414.1(2008):399-406. |
入库方式： OAI收割
来源：上海营养与健康研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。