Detection of constitutive heterodimerization of the integrin Mac-1 subunits by fluorescence resonance energy transfer in living cells
文献类型：期刊论文
| 作者 | Fu Guo ; Yang Hua-Yan ; Wang Chen ; Zhang Feng ; You Zhen-Dong ; Wang Gui-Ying ; He Cheng ; Chen Yi-Zhang ; Xu ZZ(徐至展) |
| 刊名 | biochem. biophys. res. commun.
 |
| 出版日期 | 2006 |
| 卷号 | 346期号:3页码:986 |
| 关键词 | FRET Mac-1 heterodimerization spectra leukocyte adhesion deficiency (LAD) FR CFP YFP in vivo CHO |
| ISSN号 | 0006-291x |
| 中文摘要 | macrophage differentiation antigen associated with complement three receptor function (mac-1) belongs to beta(2) subfamily of integrins that mediate important cell-cell and cell-extracellular matrix interactions. biochemical studies have indicated that mac-1 is a constitutive heterodimer in vitro. here, we detected the heterodimerization of mac-1 subunits in living cells by means of two fluorescence resonance energy transfer (fret) techniques (fluorescence microscopy and fluorescence spectroscopy) and our results demonstrated that there is constitutive heterodimerization of the mac-1 subunits and this constitutive heterodimerization of the mac-1 subunits is cell-type independent. through fret imaging, we found that heterodimers of mac-1 mainly localized in plasma membrane, perinuclear, and golgi area in living cells. furthermore, through analysis of the estimated physical distances between cyan fluorescent protein (cfp) and yellow fluorescent protein (yfp) fused to mac-1 subunits, we suggested that the conformation of mac-1 subunits is not affected by the fusion of cfp or yfp and inferred that mac-1 subunits take different conformation when expressed in chinese hamster ovary (cho) and human embryonic kidney (hek) 293t cells, respectively. (c) 2006 elsevier inc. all rights reserved. |
| 收录类别 | 0 |
| 语种 | 英语 |
| WOS记录号 | WOS:000238865900048 |
| 公开日期 | 2009-09-18 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/802]  |
| 专题 | 上海光学精密机械研究所_强场激光物理国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Fu Guo,Yang Hua-Yan,Wang Chen,et al. Detection of constitutive heterodimerization of the integrin Mac-1 subunits by fluorescence resonance energy transfer in living cells[J]. biochem. biophys. res. commun.,2006,346(3):986, 991. |
| APA | Fu Guo.,Yang Hua-Yan.,Wang Chen.,Zhang Feng.,You Zhen-Dong.,...&徐至展.(2006).Detection of constitutive heterodimerization of the integrin Mac-1 subunits by fluorescence resonance energy transfer in living cells.biochem. biophys. res. commun.,346(3),986. |
| MLA | Fu Guo,et al."Detection of constitutive heterodimerization of the integrin Mac-1 subunits by fluorescence resonance energy transfer in living cells".biochem. biophys. res. commun. 346.3(2006):986. |
入库方式： OAI收割
来源：上海光学精密机械研究所
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