Detection of constitutive homomeric associations of the integrins Mac-1 subunits by fluorescence resonance energy transfer in living cells
文献类型：期刊论文
| 作者 | Fu Guo ; Wang Chen ; Wang Gui-ying ; Chen Yi-zhang ; He Cheng ; Xu ZZ(徐至展) |
| 刊名 | biochem. biophys. res. commun.
 |
| 出版日期 | 2006 |
| 卷号 | 351期号:4页码:847 |
| 关键词 | FRET Mac-1 homomeric association spectra subunit FR CFP YFP in vivo CHO |
| ISSN号 | 0006-291x |
| 中文摘要 | integrins alpha(m)beta(2) plays important role on leukocytes, such as adhesion, migration, phagocytosis, and apoptosis. it was hypothesized that homomeric associations of integrin subunits provide a driving force for integrins activation, and simultaneously inducing the formation of integrins clusters. however, experimental reports on homomeric associations between integrin subunits are still controversial. here, we proved the homomeric associations of the isolated mac-1 subunits in living cells using three-channel fluorescence resonance energy transfer (fret) microscopy and fret spectra methods. we found that the extent of homomeric associations between beta(2) subunits is higher than alpha(m) subunits. furthermore, fret imaging indicated that the extent of homomeric associations of the mac-1 subunits is higher along the plasma membrane than in the cytoplasm. finally, we suggested that homomeric associations of the transmernbrane domains or/and cytoplasmic domains may provide the driving force for the formation of constitutive homomeric associations between alpha(m) or beta(2) subunits. (c) 2006 elsevier inc. all rights reserved. |
| 收录类别 | 0 |
| 语种 | 英语 |
| WOS记录号 | WOS:000242425300009 |
| 公开日期 | 2009-09-18 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/804]  |
| 专题 | 上海光学精密机械研究所_强场激光物理国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Fu Guo,Wang Chen,Wang Gui-ying,et al. Detection of constitutive homomeric associations of the integrins Mac-1 subunits by fluorescence resonance energy transfer in living cells[J]. biochem. biophys. res. commun.,2006,351(4):847, 852. |
| APA | Fu Guo,Wang Chen,Wang Gui-ying,Chen Yi-zhang,He Cheng,&徐至展.(2006).Detection of constitutive homomeric associations of the integrins Mac-1 subunits by fluorescence resonance energy transfer in living cells.biochem. biophys. res. commun.,351(4),847. |
| MLA | Fu Guo,et al."Detection of constitutive homomeric associations of the integrins Mac-1 subunits by fluorescence resonance energy transfer in living cells".biochem. biophys. res. commun. 351.4(2006):847. |
入库方式： OAI收割
来源：上海光学精密机械研究所
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