The Neddylation-Cullin 2-RBX1 E3 Ligase Axis Targets Tumor Suppressor RhoB for Degradation in Liver Cancer
文献类型：期刊论文
| 作者 | Xu, Junfeng; Li, Lihui; Yu, Guangyang; Ying, Wantao; Gao, Qiang; Zhang, Wenjuan; Li, Xianyu; Ding, Chen; Jiang, Yanan; Wei, Dongping |
| 刊名 | MOLECULAR & CELLULAR PROTEOMICS
 |
| 出版日期 | 2015 |
| 卷号 | 14期号:3页码:499-509 |
| 英文摘要 | recently been identified as a potential oncogenic event and attractive anticancer target; however, its underlying mechanisms have not been well elucidated. In this study, RhoB, a well known tumor suppressor, was identified and validated with an iTRAQ-based quantitative proteomic approach as a new target of this pathway in liver cancer cells. Specifically, cullin 2-RBX1 E3 ligase, which requires NEDD8 conjugation for its activation, interacted with RhoB and promoted its ubiquitination and degradation. In human liver cancer tissues, the neddylation-CRL pathway was overactivated and reversely correlated with RhoB levels. Moreover, RhoB accumulation upon inhibition of the neddylation-CRL pathway for anticancer therapy contributed to the induction of tumor suppressors p21 and p27, apoptosis, and growth suppression. Our findings highlight the degradation of RhoB via the neddylation-CRL pathway as an important molecular event that drives liver carcinogenesis and RhoB itself as a pivotal effector for anticancer therapy targeting this oncogenic pathway. |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | NEDD8-ACTIVATING ENZYME-INHIBITOR ; UBIQUITIN LIGASE ; CONJUGATING ENZYMES ; PROTEIN-DEGRADATION ; ANTICANCER THERAPY ; NEDD8 CONJUGATION ; ENDOTHELIAL-CELLS ; HIF-ALPHA ; EXPRESSION ; GROWTH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000350671300005 |
| 公开日期 | 2015-09-08 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/135]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_核受体与代谢和疾病研究组 |
| 推荐引用方式 GB/T 7714 | Xu, Junfeng,Li, Lihui,Yu, Guangyang,et al. The Neddylation-Cullin 2-RBX1 E3 Ligase Axis Targets Tumor Suppressor RhoB for Degradation in Liver Cancer[J]. MOLECULAR & CELLULAR PROTEOMICS,2015,14(3):499-509. |
| APA | Xu, Junfeng.,Li, Lihui.,Yu, Guangyang.,Ying, Wantao.,Gao, Qiang.,...&Jia, Lijun.(2015).The Neddylation-Cullin 2-RBX1 E3 Ligase Axis Targets Tumor Suppressor RhoB for Degradation in Liver Cancer.MOLECULAR & CELLULAR PROTEOMICS,14(3),499-509. |
| MLA | Xu, Junfeng,et al."The Neddylation-Cullin 2-RBX1 E3 Ligase Axis Targets Tumor Suppressor RhoB for Degradation in Liver Cancer".MOLECULAR & CELLULAR PROTEOMICS 14.3(2015):499-509. |
入库方式： OAI收割
来源：上海营养与健康研究所
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