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Genetic targeting of sprouting angiogenesis using Apln-CreER
文献类型：期刊论文
| 作者 | Liu, Qiaozhen; Hu, Tianyuan; He, Lingjuan; Huang, Xiuzhen; Tian, Xueying; Zhang, Hui; He, Liang; Pu, Wenjuan; Zhang, Libo; Sun, Heng |
| 刊名 | Nature Communications
 |
| 出版日期 | 2015 |
| 卷号 | 6期号:1页码:6020 |
| 英文摘要 | Under pathophysiological conditions in adults, endothelial cells (ECs) sprout from pre-existing blood vessels to form new ones by a process termed angiogenesis. During embryonic development, Apelin (APLN) is robustly expressed in vascular ECs. In adult mice, however, APLN expression in the vasculature is significantly reduced. Here we show that APLN expression is reactivated in adult ECs after ischaemia insults. In models of both injury ischaemia and tumor angiogenesis, we find that Apln-CreER genetically labels sprouting but not quiescent vasculature. By leveraging this specific activity, we demonstrate that abolishment of the VEGF-VEGFR2 signalling pathway as well as ablation of sprouting ECs diminished tumour vascularization and growth without compromising vascular homeostasis in other organs. Collectively, we show that Apln-CreER distinguishes sprouting vessels from stabilized vessels in multiple pathological settings. The Apln-CreER line described here will greatly aid future mechanistic studies in both vascular developmental biology and adult vascular diseases. |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | BLOOD MONONUCLEAR-CELLS ; ENDOTHELIAL-CELLS ; IN-VIVO ; PATHOLOGICAL ANGIOGENESIS ; TUMOR ANGIOGENESIS ; TRANSGENIC MOUSE ; LINEAGE ANALYSIS ; APELIN ; VEGF ; HEART |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000348829600005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/137]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_核受体与代谢和疾病研究组 中国科学院上海生命科学研究院营养科学研究所_营养与癌症研究组 中国科学院上海生命科学研究院营养科学研究所_磷脂代谢与心血管疾病研究组 中国科学院上海生命科学研究院营养科学研究所_心脏发育与再生研究组 |
| 推荐引用方式 GB/T 7714 | Liu, Qiaozhen,Hu, Tianyuan,He, Lingjuan,et al. Genetic targeting of sprouting angiogenesis using Apln-CreER[J]. Nature Communications,2015,6(1):6020. |
| APA | Liu, Qiaozhen.,Hu, Tianyuan.,He, Lingjuan.,Huang, Xiuzhen.,Tian, Xueying.,...&Zhou, Bin.(2015).Genetic targeting of sprouting angiogenesis using Apln-CreER.Nature Communications,6(1),6020. |
| MLA | Liu, Qiaozhen,et al."Genetic targeting of sprouting angiogenesis using Apln-CreER".Nature Communications 6.1(2015):6020. |
入库方式： OAI收割
来源：上海营养与健康研究所
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