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Smooth muscle protein 22 alpha-Cre is expressed in myeloid cells in mice

文献类型：期刊论文

作者Shen, Zhuxia; Li, Chao; Frieler, Ryan A.; Gerasimova, Alena S.; Lee, Soo Jung; Wu, Jing; Wang, Michael M.; Lumeng, Carey N.; Brosius, Frank C., III; Duan, ShengZhong（段胜仲）
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
出版日期2012
卷号422期号:4页码:639-642
关键词SM22 alpha-Cre Smooth muscle cells Myeloid cells Neutrophils Monocytes Macrophages
英文摘要Background: Experiments using Cre recombinase to study smooth muscle specific functions rely on strict specificity of Cre transgene expression. Therefore, accurate determination of Cre activity is critical to the interpretation of experiments using smooth muscle specific Cre. Methods and results: Two lines of smooth muscle protein 22 alpha-Cre (SM22 alpha-Cre) mice were bred to foxed mice in order to define Cre transgene expression. Southern blotting demonstrated that SM22 alpha-Cre was expressed not only in tissues abundant of smooth muscle, but also in spleen, which consists largely of immune cells including myeloid and lymphoid cells. PCR detected SM22 alpha-Cre expression in peripheral blood and peritoneal macrophages. Analysis of SM22 alpha-Cre mice crossed with a recombination detector GFP mouse revealed GFP expression, and hence recombination, in circulating neutrophils and monocytes by flow cytometry. Conclusions: SM22 alpha-Cre mediates recombination not only in smooth muscle cells, but also in myeloid cells including neutrophils, monocytes, and macrophages. Given the known contributions of myeloid cells to cardiovascular phenotypes, caution should be taken when interpreting data using SM22 alpha-Cre mice to investigate smooth muscle specific functions. Strategies such as bone marrow transplantation may be necessary when SM22 alpha-Cre is used to differentiate the contribution of smooth muscle cells versus myeloid cells to observed phenotypes. (C) 2012 Elsevier Inc. All rights reserved.
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]ACTIVATED RECEPTOR-GAMMA ; SOMATIC MUTAGENESIS ; TRANSGENIC MICE ; ATHEROSCLEROSIS ; HYPERTENSION ; INFLAMMATION ; RECOMBINASE ; VASCULATURE ; MECHANISMS ; DISEASE
收录类别SCI
语种英语
WOS记录号WOS:000306443900019
公开日期2015-09-08
版本出版稿
源URL[http://202.127.25.144/handle/331004/141]  
专题中国科学院上海生命科学研究院营养科学研究所_核受体与代谢和疾病研究组
推荐引用方式
GB/T 7714		 Shen, Zhuxia,Li, Chao,Frieler, Ryan A.,et al. Smooth muscle protein 22 alpha-Cre is expressed in myeloid cells in mice[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2012,422(4):639-642.
APA Shen, Zhuxia.,Li, Chao.,Frieler, Ryan A..,Gerasimova, Alena S..,Lee, Soo Jung.,...&Mortensen, Richard M..(2012).Smooth muscle protein 22 alpha-Cre is expressed in myeloid cells in mice.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,422(4),639-642.
MLA Shen, Zhuxia,et al."Smooth muscle protein 22 alpha-Cre is expressed in myeloid cells in mice".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 422.4(2012):639-642.

入库方式： OAI收割

来源：上海营养与健康研究所

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