Effects of PPARs Agonists on Cardiac Metabolism in Littermate and Cardiomyocyte-Specific PPAR-gamma - Knockout (CM-PGKO) Mice
文献类型：期刊论文
| 作者 | Barbieri, Michelangela; Di Filippo, Clara; Esposito, Antonietta; Marfella, Raffaele; Rizzo, Maria Rosaria; D'Amico, Michele; Ferraraccio, Franca; Di Ronza, Cristina; Duan, ShengZhong（段胜仲） ; Mortensen, Richard M.
|
| 刊名 | PLOS ONE
 |
| 出版日期 | 2012 |
| 卷号 | 7期号:4页码:e35999 |
| 英文摘要 | Understanding the molecular regulatory mechanisms controlling for myocardial lipid metabolism is of critical importance for the development of new therapeutic strategies for heart diseases. The role of PPAR gamma and thiazolidinediones in regulation of myocardial lipid metabolism is controversial. The aim of our study was to assess the role of PPAR gamma on myocardial lipid metabolism and function and differentiate local/from systemic actions of PPARs agonists using cardiomyocyte-specific PPAR gamma -knockout (CM-PGKO) mice. To this aim, the effect of PPAR gamma, PPAR gamma/PPAR alpha and PPAR alpha agonists on cardiac function, intra-myocyte lipid accumulation and myocardial expression profile of genes and proteins, affecting lipid oxidation, uptake, synthesis, and storage (CD36, CPT1MIIA, AOX, FAS, SREBP1-c and ADPR) was evaluated in cardiomyocyte-specific PPAR gamma -knockout (CM-PGKO) and littermate control mice undergoing standard and high fat diet (HFD). At baseline, protein levels and mRNA expression of genes involved in lipid uptake, oxidation, synthesis, and accumulation of CM-PGKO mice were not significantly different from those of their littermate controls. At baseline, no difference in myocardial lipid content was found between CM-PGKO and littermate controls. In standard condition, pioglitazone and rosiglitazone do not affect myocardial metabolism while, fenofibrate treatment significantly increased CD36 and CPT1MIIA gene expression. In both CM-PGKO and control mice submitted to HFD, six weeks of treatment with rosiglitazone, fenofibrate and pioglitazone lowered myocardial lipid accumulation shifting myocardial substrate utilization towards greater contribution of glucose. In conclusion, at baseline, PPAR gamma does not play a crucial role in regulating cardiac metabolism in mice, probably due to its low myocardial expression. PPARs agonists, indirectly protect myocardium from lipotoxic damage likely reducing fatty acids delivery to the heart through the actions on adipose tissue. Nevertheless a direct non- PPAR gamma mediated mechanism of PPAR gamma agonist could not be ruled out. |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | ACTIVATED-RECEPTOR-GAMMA ; MYOCARDIAL-INFARCTION ; LIPOTOXIC CARDIOMYOPATHY ; QUANTITATIVE-ANALYSIS ; LIPID-ACCUMULATION ; HEART-FAILURE ; RAT-HEART ; ROSIGLITAZONE ; HYPERTROPHY ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000305349100058 |
| 公开日期 | 2015-09-08 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/142]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_核受体与代谢和疾病研究组 |
| 推荐引用方式 GB/T 7714 | Barbieri, Michelangela,Di Filippo, Clara,Esposito, Antonietta,et al. Effects of PPARs Agonists on Cardiac Metabolism in Littermate and Cardiomyocyte-Specific PPAR-gamma - Knockout (CM-PGKO) Mice[J]. PLOS ONE,2012,7(4):e35999. |
| APA | Barbieri, Michelangela.,Di Filippo, Clara.,Esposito, Antonietta.,Marfella, Raffaele.,Rizzo, Maria Rosaria.,...&Paolisso, Giuseppe.(2012).Effects of PPARs Agonists on Cardiac Metabolism in Littermate and Cardiomyocyte-Specific PPAR-gamma - Knockout (CM-PGKO) Mice.PLOS ONE,7(4),e35999. |
| MLA | Barbieri, Michelangela,et al."Effects of PPARs Agonists on Cardiac Metabolism in Littermate and Cardiomyocyte-Specific PPAR-gamma - Knockout (CM-PGKO) Mice".PLOS ONE 7.4(2012):e35999. |
入库方式： OAI收割
来源：上海营养与健康研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。