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A quantitative protocol for rapid analysis of cell density and size distribution of pelagic and benthic Microcystis colonies by FlowCAM

文献类型：期刊论文

作者Wang, Chunbo1,2; Wu, Xingqiang1; Tian, Cuicui1,2; Li, Qian1,2; Tian, Yingying1,2; Feng, Bing1,2; Xiao, Bangding1
刊名JOURNAL OF APPLIED PHYCOLOGY
出版日期2015-04-01
卷号27期号:2页码:711-720
关键词Microcystis Cell density Size distribution FlowCAM Aalkaline hydrolysis
ISSN号0921-8971
通讯作者Xiao, BD (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
英文摘要Colony formation plays an important role in the life history of Microcystis. However, analyzing the colony size distribution with a microscope is time consuming, and colony formation also hinders the direct monitoring of cell density. In this study, a quantitative protocol for rapidly analyzing the cell density and colony size distribution of pelagic and benthic Microcystis was developed. Microcystis colonies were disintegrated by alkaline hydrolysis with 0.01-0.05 mol L-1 sodium hydroxide at 85 A degrees C for 6-8 min and automatically measured by the Flow Camera And Microscope (FlowCAM). Benthic Microcystis colonies were isolated from different lake sediments using 40 % Percoll solution. Alkaline hydrolysis was validated as a rapid and universally effective method to disintegrate different morphospecies of Microcystis colonies. The FlowCAM exhibited excellent accuracy, reproducibility, and efficiency in determining the cell density and size distribution of Microcystis. The developed protocol was successfully applied to field samples from Lake Caohai (China) and can accurately monitor the population dynamics and colony size distribution of bloom-forming Microcystis.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
研究领域[WOS]Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
关键词[WOS]BLOOM-FORMING MICROCYSTIS ; CYANOBACTERIUM MICROCYSTIS ; LAKE-ERIE ; AERUGINOSA ; CHINA ; PHYTOPLANKTON ; ENUMERATION ; DIVERSITY ; TAIHU ; CYANOPHYCEAE
收录类别SCI
语种英语
WOS记录号WOS:000352446200009
源URL[http://ir.ihb.ac.cn/handle/342005/23944]  
专题水生生物研究所_水环境工程研究中心_期刊论文
作者单位1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 10085, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Chunbo,Wu, Xingqiang,Tian, Cuicui,et al. A quantitative protocol for rapid analysis of cell density and size distribution of pelagic and benthic Microcystis colonies by FlowCAM[J]. JOURNAL OF APPLIED PHYCOLOGY,2015,27(2):711-720.
APA Wang, Chunbo.,Wu, Xingqiang.,Tian, Cuicui.,Li, Qian.,Tian, Yingying.,...&Xiao, Bangding.(2015).A quantitative protocol for rapid analysis of cell density and size distribution of pelagic and benthic Microcystis colonies by FlowCAM.JOURNAL OF APPLIED PHYCOLOGY,27(2),711-720.
MLA Wang, Chunbo,et al."A quantitative protocol for rapid analysis of cell density and size distribution of pelagic and benthic Microcystis colonies by FlowCAM".JOURNAL OF APPLIED PHYCOLOGY 27.2(2015):711-720.

入库方式： OAI收割

来源：水生生物研究所

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