中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Perfluorooctyl Iodide Stimulates Steroidogenesis in H295R Cells via a Cyclic Adenosine Monophosphate Signaling Pathway

文献类型:期刊论文

作者Wang, Chang1,2; Ruan, Ting2; Liu, Jiyan2; He, Bin2; Zhou, Qunfang2; Jiang, Guibin2
刊名CHEMICAL RESEARCH IN TOXICOLOGY
出版日期2015-05-01
卷号28期号:5页码:848-854
ISSN号0893-228X
通讯作者Jiang, GB (reprint author), Chinese Acad Sci, Ecoenvironm Sci Res Ctr, POB 2871, Beijing 100085, Peoples R China.
英文摘要Perfluorinated iodine alkalies (PFIs) are used widely in the organic fluorine industry. Increased production of PFIs has caused environmental health concern. To evaluate the potential endocrine-disrupting effect of PFIs, we investigated the effects of perfluorooctyl iodide (PFOI) on steroidogenesis in human adrenocortical carcinoma cells (H295R). Levels of aldosterone, 17 beta-estradiol, and testosterone,were measured in H295R culture medium upon treatment with perfluorooctanoic acid (PFOA) and PFIs. Expression of 10 steroidogenic genes (StAR, HMGR, CYP11A1 3 beta HSD2, 17 beta HSD; CYP17, CYP21, CYP11B1, CYP11B2, and CYP19) was measured by-real-time polymerase chain reaction. Levels of cyclic adenosine monophosphate (cAMP) and adenylate cyclase (AC) activity were measured to understand the underlying mechanism of steroidogenic perturbations. Levels of production of aldosterone, cortisol, and 17 beta-estradiol were elevated significantly, and the level of testosterone generation decreased upon treatment with 100 mu M PFOI. Similar to the, effect induced by forskolin (AC activator), expression of all 10 genes involved in the synthesis of steroid hormones was upregulated significantly upon exposure to 100 mu M PFOI. PFOA had no effect On steroid hormone production or steroidogenic gene expression even though it is highly structurally similar with PFOI. Therefore, the terminal -CF2I roup in PFOI could-be a critical factor for mediation of steroidogenesis. PFOI increased AC activity and cAMP levels in H295R cells, which implied an underlying mechanism for the disturbance of steroidogenesis. These data suggest that PFOI may act as an AC activator,, thereby stimulating steroidogenesis by activating a cAMP signaling pathway.
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Toxicology
研究领域[WOS]Pharmacology & Pharmacy ; Chemistry ; Toxicology
关键词[WOS]PERFLUORINATED CARBOXYLIC-ACIDS ; STEROID-HORMONE BIOSYNTHESIS ; ACUTE REGULATORY PROTEIN ; MEDAKA ORYZIAS-LATIPES ; IN-VITRO ; FLUOROTELOMER ALCOHOLS ; VITELLOGENIN INDUCTION ; ESTROGENIC ACTIVITIES ; HEPATIC VITELLOGENIN ; PBDE METABOLITES
收录类别SCI
语种英语
WOS记录号WOS:000354907500003
源URL[http://ir.ihb.ac.cn/handle/342005/23964]  
专题水生生物研究所_水环境工程研究中心_期刊论文
作者单位1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
推荐引用方式
GB/T 7714
Wang, Chang,Ruan, Ting,Liu, Jiyan,et al. Perfluorooctyl Iodide Stimulates Steroidogenesis in H295R Cells via a Cyclic Adenosine Monophosphate Signaling Pathway[J]. CHEMICAL RESEARCH IN TOXICOLOGY,2015,28(5):848-854.
APA Wang, Chang,Ruan, Ting,Liu, Jiyan,He, Bin,Zhou, Qunfang,&Jiang, Guibin.(2015).Perfluorooctyl Iodide Stimulates Steroidogenesis in H295R Cells via a Cyclic Adenosine Monophosphate Signaling Pathway.CHEMICAL RESEARCH IN TOXICOLOGY,28(5),848-854.
MLA Wang, Chang,et al."Perfluorooctyl Iodide Stimulates Steroidogenesis in H295R Cells via a Cyclic Adenosine Monophosphate Signaling Pathway".CHEMICAL RESEARCH IN TOXICOLOGY 28.5(2015):848-854.

入库方式: OAI收割

来源:水生生物研究所

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