Physicochemical Evolution and Molecular Adaptation of the Cetacean Osmoregulation-related Gene UT-A2 and Implications for Functional Studies
文献类型：期刊论文
| 作者 | Wang, Jingzhen1,2,3; Yu, Xueying1,2,4; Hu, Bo1,2; Zheng, Jinsong1,2; Xiao, Wuhan1,2; Hao, Yujiang1,2; Liu, Wenhua3; Wang, Ding1,2 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2015-03-12 |
| 卷号 | 5 |
| ISSN号 | 2045-2322 |
| 通讯作者 | Liu, WH (reprint author), Shantou Univ, Inst Marine Biol, Shantou 515063, Guangdong, Peoples R China. |
| 英文摘要 | Cetaceans have an enigmatic evolutionary history of re-invading aquatic habitats. One of their essential adaptabilities that has enabled this process is their homeostatic strategy adjustment. Here, we investigated the physicochemical evolution and molecular adaptation of the cetacean urea transporter UT-A2, which plays an important role in urine concentration and water homeostasis. First, we cloned UT-A2 from the freshwater Yangtze finless porpoise, after which bioinformatics analyses were conducted based on available datasets (including freshwater baiji and marine toothed and baleen whales) using MEGA, PAML, DataMonkey, TreeSAAP and Consurf. Our findings suggest that the UT-A2 protein shows folding similar to that of dvUT and UT-B, whereas some variations occurred in the functional S-o and S-i regions of the selectivity filter. Additionally, several regions of the cetacean UT-A2 protein have experienced molecular adaptations. We suggest that positive-destabilizing selection could contribute to adaptations by influencing its biochemical and conformational character. The conservation of amino acid residues within the selectivity filter of the urea conduction pore is likely to be necessary for urea conduction, whereas the non-conserved amino acid replacements around the entrance and exit of the conduction pore could potentially affect the activity, which could be interesting target sites for future mutagenesis studies. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | REGULATED UREA TRANSPORTER ; AMINO-ACID SITES ; URINE-CONCENTRATING MECHANISM ; DOLPHINS TURSIOPS-TRUNCATUS ; GENOME-WIDE ASSOCIATION ; HELICOBACTER-PYLORI ; POSITIVE SELECTION ; MARINE MAMMALS ; RAT-KIDNEY ; ADAPTIVE EVOLUTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351142200001 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/23835]  |
| 专题 | 水生生物研究所_水生生物多样性与资源保护研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 3.Shantou Univ, Inst Marine Biol, Shantou 515063, Guangdong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jingzhen,Yu, Xueying,Hu, Bo,et al. Physicochemical Evolution and Molecular Adaptation of the Cetacean Osmoregulation-related Gene UT-A2 and Implications for Functional Studies[J]. SCIENTIFIC REPORTS,2015,5. |
| APA | Wang, Jingzhen.,Yu, Xueying.,Hu, Bo.,Zheng, Jinsong.,Xiao, Wuhan.,...&Wang, Ding.(2015).Physicochemical Evolution and Molecular Adaptation of the Cetacean Osmoregulation-related Gene UT-A2 and Implications for Functional Studies.SCIENTIFIC REPORTS,5. |
| MLA | Wang, Jingzhen,et al."Physicochemical Evolution and Molecular Adaptation of the Cetacean Osmoregulation-related Gene UT-A2 and Implications for Functional Studies".SCIENTIFIC REPORTS 5(2015). |
入库方式： OAI收割
来源：水生生物研究所
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