Identification of a germ cell marker gene, the dead end homologue, in Chinese sturgeon Acipenser sinensis
文献类型：期刊论文
| 作者 | Yang Xiaoge1,2; Yue Huamei2,3; Ye Huan1,2; Li Chuangju2,3; Wei Qiwei1,2,3 |
| 刊名 | GENE
 |
| 出版日期 | 2015-03-01 |
| 卷号 | 558期号:1页码:118-125 |
| 关键词 | Dead end Fluorescent in situ hybridization Primordial germ cell qRT-PCR Chinese sturgeon |
| ISSN号 | 0378-1119 |
| 通讯作者 | Li, CJ (reprint author), Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, 8 1st Wudayuan Rd, Wuhan 430223, Peoples R China. |
| 英文摘要 | Dead end (dnd) encodes an RNA-binding protein that is essential for primordial germ cell (PGC) migration and gametogenesis in vertebrates. In this study, a Chinese sturgeon Acipenser sinensis dead end homologue, designated Asdnd, was identified and characterized. The full-length cDNA of Asdnd was 1630 base pairs (bp) and encoded a peptide of 396 amino acid residues. Multiple sequence alignment showed that AsDnd shared six conserved regions of Dnd orthologs, including the RNA recognition motif. Phylogenetic analysis revealed that AsDnd was grouped with teleosts. By quantitative real-time PCR analysis, the Asdnd transcripts were found to originate from the maternal parent and be specifically expressed in gonads of immature Chinese sturgeons of both sexes. Fluorescent in situ hybridization indicated thatAsdnd transcripts were restricted to germ cells. In the testis, Asdnd was abundant in spermatogonia and tended to gradually diminish in late spermatogenic stages, while strong signals were found in primary oocytes, as opposed to oogonia, in the ovary. Zebrafish PGCs were clearly visualized at 24 h post-fertilization by co-injecting RFP-Asdnd 3' UTR and GFP-nos3 3' UTR mRNA, indicating that dnd 3' UTR has a conserved function among teleosts. Therefore, dnd could serve as a germ cell marker in Chinese sturgeon. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Genetics & Heredity |
| 研究领域[WOS] | Genetics & Heredity |
| 关键词[WOS] | MESSENGER-RNA ; ZEBRAFISH MIR-430 ; EXPRESSION ; CLEARANCE ; MIGRATION ; SOMA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000349505100014 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/23839]  |
| 专题 | 水生生物研究所_水生生物多样性与资源保护研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Fishery Sci, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China 3.Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang Xiaoge,Yue Huamei,Ye Huan,et al. Identification of a germ cell marker gene, the dead end homologue, in Chinese sturgeon Acipenser sinensis[J]. GENE,2015,558(1):118-125. |
| APA | Yang Xiaoge,Yue Huamei,Ye Huan,Li Chuangju,&Wei Qiwei.(2015).Identification of a germ cell marker gene, the dead end homologue, in Chinese sturgeon Acipenser sinensis.GENE,558(1),118-125. |
| MLA | Yang Xiaoge,et al."Identification of a germ cell marker gene, the dead end homologue, in Chinese sturgeon Acipenser sinensis".GENE 558.1(2015):118-125. |
入库方式： OAI收割
来源：水生生物研究所
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