Preparation of monoPEGylated Cyanovirin-N's derivative and its anti-influenza A virus bioactivity in vitro and in vivo
文献类型：期刊论文
| 作者 | Wu, Chongchao1,2; Chen, Wei1; Chen, Jia1; Han, Bo1,2; Peng, Zhou3; Ge, Feng1,4; Wei, Bo1; Liu, Mingxian1; Zhang, Meiying1; Qian, Chuiwen1 |
| 刊名 | JOURNAL OF BIOCHEMISTRY
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| 出版日期 | 2015-06-01 |
| 卷号 | 157期号:6页码:539-548 |
| ISSN号 | 0021-924X |
| 关键词 | Cyanovirin-N influenza A virus mice PEGylation polypeptide linker |
| 通讯作者 | Xiong, S (reprint author), Jinan Univ, Biomed R&D Ctr, 5-F,Bldg Biol, Guangzhou 510632, Guangdong, Peoples R China. |
| 英文摘要 | Influenza A virus (IAV) has been raising public health and safety concerns worldwide. Cyanovirin-N (CVN) is a prominent anti-IAV candidate, but both cytotoxicity and immunogenicity have hindered the development of this protein as a viable therapy. In this article, linker-CVN (LCVN) with a flexible and hydrophilic polypeptide at the N-terminus was efficiently produced from the cytoplasm of Escherichia coli at a > 15-l scale. PEGylation at the N-terminal alpha-amine of LCVN was also reformed as 20 kDa PEGylated linkered Cyanovirin-N (PEG(20k)-LCVN). The 50% effective concentrations of PEG(20k)-LCVN were 0.43 +/- 0.11 A mu M for influenza A/HK/8/68 (H3N2) and 0.04 +/- 0.02 A mu M for A/Swan/Hokkaido/51/96 (H5N3), dramatically lower than that of the positive control, Ribavirin (2.88 +/- 0.66 x 10(3) A mu M and 1.79 +/- 0.62 x 10(3) A mu M, respectively). A total of 12.5 A mu M PEG(20k)-LCVN effectively inactivate the propagation of H3N2 in chicken embryos. About 2.0 mg/kg/day PEG(20k)-LCVN increased double the survival rate (66.67%, P = 0.0378) of H3N2 infected mice, prolonged the median survival period, downregulated the mRNA level of viral nuclear protein and decreased (attenuated) the pathology lesion in mice lung. A novel PEGylated CVN derivative, PEG(20k)-LCVN, exhibited potent and strain-dependent anti-IAV activity in nanomolar concentrations in vitro, as well as in micromolar concentration in vivo. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | TRANSFERRIN FUSION PROTEIN ; COLONY-STIMULATING FACTOR ; MICROBICIDE DEVELOPMENT ; INACTIVATING PROTEIN ; INFLUENZA VACCINES ; PEGYLATION ; EXPRESSION ; EFFICACY ; HIV |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000356238100013 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/23969]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Jinan Univ, Inst Biomedicine Natl Engn Res Ctr Genet Med, Dept Cellular Biol, Guangzhou 510632, Guangdong, Peoples R China 2.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Minist Educ, Key Lab Syst Biomed, Shanghai 200240, Peoples R China 3.Zhejiang Ocean Univ, Dept Pharm, Coll Food & Pharm Med, Zhoushan 316002, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 5.Nagasaki Univ, Grad Sch Biomed Sci, Dept Mol Microbiol & Immunol, Div Mol Pharmacol Infect Agents, Nagasaki, Nagasaki Prefec 8528521, Japan 6.Univ Pittsburgh, Dept Med, Pittsburgh, PA 15213 USA |
| 推荐引用方式 GB/T 7714 | Wu, Chongchao,Chen, Wei,Chen, Jia,et al. Preparation of monoPEGylated Cyanovirin-N's derivative and its anti-influenza A virus bioactivity in vitro and in vivo[J]. JOURNAL OF BIOCHEMISTRY,2015,157(6):539-548. |
| APA | Wu, Chongchao.,Chen, Wei.,Chen, Jia.,Han, Bo.,Peng, Zhou.,...&Xiong, Sheng.(2015).Preparation of monoPEGylated Cyanovirin-N's derivative and its anti-influenza A virus bioactivity in vitro and in vivo.JOURNAL OF BIOCHEMISTRY,157(6),539-548. |
| MLA | Wu, Chongchao,et al."Preparation of monoPEGylated Cyanovirin-N's derivative and its anti-influenza A virus bioactivity in vitro and in vivo".JOURNAL OF BIOCHEMISTRY 157.6(2015):539-548. |
入库方式： OAI收割
来源：水生生物研究所
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