ATP-binding cassette transporter enhances tolerance to DDT in Tetrahymena
文献类型：期刊论文
| 作者 | Ning YingZhi1; Dang Huai1; Liu GuangLong1; Xiong Jie2; Yuan DongXia2; Feng LiFang3; Miao Wei2 |
| 刊名 | SCIENCE CHINA-LIFE SCIENCES
 |
| 出版日期 | 2015-03-01 |
| 卷号 | 58期号:3页码:297-304 |
| ISSN号 | 1674-7305 |
| 关键词 | Tetrahymena DDT ATP-binding cassette transporter tolerance |
| 通讯作者 | Feng, LF (reprint author), Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Key Lab Food Safety Zhejiang Prov, Hangzhou 310018, Peoples R China. |
| 英文摘要 | The reuse of dichlorodiphenyltrichloroethane (DDT) as an indoor residual spray was permitted by the World Health Organization in 2007, and approximately 14 countries still use DDT to control disease vectors. The extensive exposure of insects to DDT has resulted in the emergence of DDT resistance, especially in mosquitoes, and the mechanism for this resistance in mosquitoes has been widely reported. Spraying can also introduce DDT directly into surface water, and DDT can subsequently accumulate in microorganisms, but the mechanism for the resistance to DDT degradation in microorganisms is unclear. Using whole-genome microarray analysis, we detected an abcb15 gene that was up-regulated in a specific manner by DDT treatment in T. thermophile. The deduced ABCB15 peptide sequence had two transmembrane domains (TMDs) and two nucleotide-binding domains (NBDs) to form the structure TMD-NBD-TMD-NBD, and each NBD contained three conserved motifs: Walker-A, C-loop, and Walker-B, which indicated the T. thermophila abcb15 was a typical ABC transporter gene. The expression of ABCB15 fused with a C-terminal green fluorescent protein was found to be on the periphery of the cell, suggesting that ABCB15 was a membrane pump protein. In addition, cells with abcb15 partially knocked down (abcb15-KD) grew slower than wild-type cells in the presence of 256 mg L-1 DDT, indicating the tolerance of abcb15-KD strain to DDT exposure was decreased. Thus, we suggest that in Tetrahymena, the membrane pump protein encoded by ABCT gene abcb15 can enhance the tolerance to DDT and protect cells from this exogenous toxin by efficiently pumping it to the extracellular space. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biology |
| 研究领域[WOS] | Life Sciences & Biomedicine - Other Topics |
| 关键词[WOS] | SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; DICHLORODIPHENYLTRICHLOROETHANE DDT ; MULTIDRUG-RESISTANCE ; ABC-TRANSPORTERS ; P-GLYCOPROTEIN ; GENE-EXPRESSION ; TRIBUTYLTIN TBT ; THERMOPHILA ; IDENTIFICATION ; TRANSFORMATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351809200010 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/23984]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China 3.Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Key Lab Food Safety Zhejiang Prov, Hangzhou 310018, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ning YingZhi,Dang Huai,Liu GuangLong,et al. ATP-binding cassette transporter enhances tolerance to DDT in Tetrahymena[J]. SCIENCE CHINA-LIFE SCIENCES,2015,58(3):297-304. |
| APA | Ning YingZhi.,Dang Huai.,Liu GuangLong.,Xiong Jie.,Yuan DongXia.,...&Miao Wei.(2015).ATP-binding cassette transporter enhances tolerance to DDT in Tetrahymena.SCIENCE CHINA-LIFE SCIENCES,58(3),297-304. |
| MLA | Ning YingZhi,et al."ATP-binding cassette transporter enhances tolerance to DDT in Tetrahymena".SCIENCE CHINA-LIFE SCIENCES 58.3(2015):297-304. |
入库方式： OAI收割
来源：水生生物研究所
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