Zebrafish Abcb4 is a potential efflux transporter of microcystin-LR
文献类型：期刊论文
| 作者 | Lu, Xing1; Long, Yong2; Sun, Rongze1; Zhou, Bolan2; Lin, Li1; Zhong, Shan1; Cui, Zongbin2 |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 167页码:35-42 |
| 关键词 | Abcb4 Efflux transporter Microcystin-LR Detoxification |
| ISSN号 | 1532-0456 |
| 通讯作者 | Zhong, S (reprint author), Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430071, Hubei, Peoples R China. |
| 英文摘要 | Microcystin-LR (MC-LR) is one of the most common microcystins (MCs), which are hepatotoxic and released into a water body during a period of cyanobacterial blooms. These toxicants can be accumulated in aquatic animals and transferred along the food chain and thus pose adverse effects on aquatic environment and public health. Zebrafish Abcb4 is reported to mediate the cellular efflux of ecotoxicologically relevant compounds including galaxolide, tonalide and phenanthrene; however, it remains unclear whether Abcb4 functions in the detoxification of MC-LR. Here, we demonstrated the role of zebrafish Abcb4 in cellular efflux of MC-LR. Transcripts of zebrafish abcb4 were detected in all of adult tissues examined. MC-LR was able to induce the expression of abcb4 gene and overexpression of Abcb4 significantly decreased the cytotoxicity and accumulation of MC-LR in LLC-PK1 cells and developing embryos. In contrast, overexpression of an Abcb4-G1177D mutant abolished its transporter function but not substrate binding activity, and sensitized LLC-PK1 cells and developing embryos to this cyanobacterial toxin. Moreover, ATPase activity in developing embryos can be induced by MC-LR. Thus, zebrafish Abcb4 plays crucial roles in cellular efflux of MC-LR and is a potential molecular marker for the monitoring of cyanobacteria contamination in the aquatic environment. (C) 2014 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology |
| 关键词[WOS] | P-GLYCOPROTEIN ; EXPRESSION ; RESISTANCE ; TOXICITY ; EMBRYOS ; CELLS ; FISH ; GENE ; CYANOBACTERIA ; MDR1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000348825300005 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/23991]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Xing,Long, Yong,Sun, Rongze,et al. Zebrafish Abcb4 is a potential efflux transporter of microcystin-LR[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2015,167:35-42. |
| APA | Lu, Xing.,Long, Yong.,Sun, Rongze.,Zhou, Bolan.,Lin, Li.,...&Cui, Zongbin.(2015).Zebrafish Abcb4 is a potential efflux transporter of microcystin-LR.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,167,35-42. |
| MLA | Lu, Xing,et al."Zebrafish Abcb4 is a potential efflux transporter of microcystin-LR".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 167(2015):35-42. |
入库方式： OAI收割
来源：水生生物研究所
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