Effect of dietary isoleucine on the immunity, antioxidant status, tight junctions and microflora in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian)
文献类型：期刊论文
| 作者 | Zhao, Juan1; Feng, Lin1,2,3; Liu, Yang1,2,3; Jiang, Weidan1,2,3; Wu, Pei1; Jiang, Jun1,2,3; Zhang, Yongan4; Zhou, Xiaoqiu1,2,3 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2014-12-01 |
| 卷号 | 41期号:2页码:663-673 |
| 关键词 | Cyprinus carpio var. Jian Isoleucine Tight junction Cytokine Nuclear factor erythroid 2 (NF-E2)/p45-related factor 2 |
| ISSN号 | 1050-4648 |
| 通讯作者 | Zhou, XQ (reprint author), Sichuan Agr Univ, Inst Anim Nutr, Yaan 625014, Sichuan, Peoples R China. |
| 英文摘要 | This study was conducted to investigate the effects of dietary isoleucine (Ile) on the immune response, antioxidant status, tight junctions, and microbial population in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian). A total of 1200 juvenile jian carp with average initial weight 6.9 +/- 0.03 g were fed semi-purified isonitrogenous diets containing 4.2 (unsupplemented control group), 7.0, 9.5, 11.9, 13.9 and 16.9 g Ile kg(-1) diet for 60 days. Results indicated that Ile supplementation decreased malondialdehyde (MDA) and protein carbonyl content, and the amounts of Escherichia coli and Aeromonas in the intestine (P < 0.05), and increased the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GR), glutathione content and the amounts of Lactobacillus and Bacillus in the intestine (P < 0.05). Furthermore, real time polymerase chain reaction revealed that relative mRNA expression of copper/zinc superoxide dismutase (Cu-ZnSOD), manganese superoxide dismutase (MnSOD), CAT, NF-E2-related factor 2 (Nrf2), p38 mitogen-activated protein kinases (p38MAPK) in the intestine were increased with increasing of dietary Ile up to a certain point (P < 0.05). Conversely, the relative mRNA expression of occludin, claudin-3, daudin-7, TNF-alpha, IL-10, Kelch-like-ECH- associated protein 1 (Keap1), extracellular signal-regulated kinase 1 (ERK1) in the intestine showed a downward trend (P < 0.05). In conclusion, dietary Ile improves intestinal immune function, antioxidant capacity and microbial population, and regulates gene expression of antioxidant enzyme, tight junctions, Nrf2, Keap1, p38 and ERK1 in the intestine of Jian carp. (C) 2014 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | FED GRADED-LEVELS ; AMINO-ACID SUPPLEMENTATION ; BODY-COMPOSITION ; ENZYME-ACTIVITIES ; DIGESTIVE CAPACITY ; BARRIER FUNCTION ; GENE-EXPRESSION ; GROWTH-PERFORMANCE ; CLAUDIN EXPRESSION ; PROTEIN EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000347017500065 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/23993]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Sichuan Agr Univ, Inst Anim Nutr, Yaan 625014, Sichuan, Peoples R China 2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Yaan 625014, Sichuan, Peoples R China 3.Sichuan Agr Univ, Minist Educ, Key Lab Anim Dis Resistance Nutr China, Yaan 625014, Sichuan, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Juan,Feng, Lin,Liu, Yang,et al. Effect of dietary isoleucine on the immunity, antioxidant status, tight junctions and microflora in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian)[J]. FISH & SHELLFISH IMMUNOLOGY,2014,41(2):663-673. |
| APA | Zhao, Juan.,Feng, Lin.,Liu, Yang.,Jiang, Weidan.,Wu, Pei.,...&Zhou, Xiaoqiu.(2014).Effect of dietary isoleucine on the immunity, antioxidant status, tight junctions and microflora in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian).FISH & SHELLFISH IMMUNOLOGY,41(2),663-673. |
| MLA | Zhao, Juan,et al."Effect of dietary isoleucine on the immunity, antioxidant status, tight junctions and microflora in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian)".FISH & SHELLFISH IMMUNOLOGY 41.2(2014):663-673. |
入库方式： OAI收割
来源：水生生物研究所
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