Characterization of Zebrafish Abcc4 as an Efflux Transporter of Organochlorine Pesticides
文献类型：期刊论文
| 作者 | Lu, Xing1; Long, Yong2; Lin, Li1; Sun, Rongze1; Zhong, Shan1; Cui, Zongbin2 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2014-12-05 |
| 卷号 | 9期号:12页码:e111664 |
| ISSN号 | 1932-6203 |
| 通讯作者 | Zhong, S (reprint author), Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430072, Hubei, Peoples R China. |
| 英文摘要 | DDT and lindane are highly toxic organochlorine pesticides and posing adverse effects on the environment and public health due to their frequent usage in developing countries. ABCC4/MRP4 is an organic anion transporter that mediates cellular efflux of a wide range of exogenous and endogenous compounds such as cyclic nucleotides and anti-cancer drugs; however, it remains unclear whether ABCC4 and its orthologs function in the detoxification of organochlorine pesticides. Here, we demonstrated the roles of zebrafish Abcc4 in cellular efflux of DDT and lindane. Zebrafish abcc4 was maternally expressed in the oocytes and its transcripts were detected in the lens, pancreas, gills, liver, intestine and bladder of developing embryos and in adult tissues examined. DDT and lindane were able to induce the expression of abcc4 gene and overexpression of Abcc4 significantly decreased the cytotoxicity and accumulation of DDT and lindane in LLC-PK1 cells and developing embryos. In contrast, overexpression of an Abcc4-G1188D mutant abolished its transporter function without effects on its substrate binding activity, and sensitized LLC-PK1 cells and developing embryos to toxic pesticides. Moreover, glutathione (GSH) was involved in the efflux of cellular pesticides and ATPase activity in developing embryos can be induced by DDT or lindane. Thus, zebrafish Abcc4 plays crucial roles in cellular efflux of organochlorine pesticides and can be used a potential molecular marker for the monitor of DDT and lindane contamination in the aquatic environment. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | MULTIDRUG-RESISTANCE PROTEINS ; DICHLORODIPHENYLTRICHLOROETHANE DDT ; DIGESTIVE GLAND ; P-GLYCOPROTEIN ; GLUTATHIONE ; MRP ; EXPRESSION ; MRP4/ABCC4 ; LINDANE ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000346907200003 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/23996]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Xing,Long, Yong,Lin, Li,et al. Characterization of Zebrafish Abcc4 as an Efflux Transporter of Organochlorine Pesticides[J]. PLOS ONE,2014,9(12):e111664. |
| APA | Lu, Xing,Long, Yong,Lin, Li,Sun, Rongze,Zhong, Shan,&Cui, Zongbin.(2014).Characterization of Zebrafish Abcc4 as an Efflux Transporter of Organochlorine Pesticides.PLOS ONE,9(12),e111664. |
| MLA | Lu, Xing,et al."Characterization of Zebrafish Abcc4 as an Efflux Transporter of Organochlorine Pesticides".PLOS ONE 9.12(2014):e111664. |
入库方式： OAI收割
来源：水生生物研究所
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