Contribution of nuclease to the pathogenesis of Aeromonas hydrophila
文献类型：期刊论文
| 作者 | Ji, Yachan1; Li, Jinquan2; Qin, Zhendong1; Li, Aihua5; Gu, Zemao1,3,4; Liu, Xiaoling1,3,4; Lin, Li1,3,4; Zhou, Yang1,3,4 |
| 刊名 | VIRULENCE
 |
| 出版日期 | 2015-07-04 |
| 卷号 | 6期号:5页码:515-522 |
| 关键词 | pathogenesis Aeromonas hydrophila nuclease |
| ISSN号 | 2150-5594 |
| 通讯作者 | Zhou, Y (reprint author), Huazhong Agr Univ, Dept Aquat Anim Med, Coll Fisheries, Wuhan, Peoples R China. |
| 英文摘要 | Aeromonas hydrophila is a gram-negative bacterium that is widely distributed in aquatic environments and can cause septicemia in both fish and humans. However, the underlying mechanisms leading to severe infection are not well understood. In this study, an A. hydrophila nuclease (ahn) deletion mutant was constructed to investigate its contribution to pathogenesis. This mutant did not differ from the wild-type strain in terms of its growth or hemolytic phenotype. However, the ahn-deficient mutant was more susceptible to being killed by fish macrophages and mouse blood in vitro. Furthermore, evidence obtained using both fish and murine infection models strongly indicated that the inactivation of Ahn impaired the ability of A. hydrophila to evade innate immune clearance in vivo. More importantly, the virulence of the mutant was attenuated in both fish and mice, with reductions in dissemination capacities and mortality rates. These findings implicate Ahn in A. hydrophila virulence, with important functions in evading innate immune defenses. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology ; Infectious Diseases ; Microbiology |
| 研究领域[WOS] | Immunology ; Infectious Diseases ; Microbiology |
| 关键词[WOS] | NEUTROPHIL EXTRACELLULAR TRAPS ; GROUP-A STREPTOCOCCUS ; CLINICAL ISOLATE ; VIRULENCE ; IDENTIFICATION ; INFECTION ; EFFECTOR ; MACROPHAGES ; ENTEROTOXIN ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000357649400018 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/23885]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Huazhong Agr Univ, Dept Aquat Anim Med, Coll Fisheries, Wuhan, Peoples R China 2.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan, Peoples R China 3.Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan, Peoples R China 4.Minist Agr, Key Lab Freshwater Anim Breeding, Wuhan, Peoples R China 5.State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji, Yachan,Li, Jinquan,Qin, Zhendong,et al. Contribution of nuclease to the pathogenesis of Aeromonas hydrophila[J]. VIRULENCE,2015,6(5):515-522. |
| APA | Ji, Yachan.,Li, Jinquan.,Qin, Zhendong.,Li, Aihua.,Gu, Zemao.,...&Zhou, Yang.(2015).Contribution of nuclease to the pathogenesis of Aeromonas hydrophila.VIRULENCE,6(5),515-522. |
| MLA | Ji, Yachan,et al."Contribution of nuclease to the pathogenesis of Aeromonas hydrophila".VIRULENCE 6.5(2015):515-522. |
入库方式： OAI收割
来源：水生生物研究所
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