MAVS splicing variants contribute to the induction of interferon and interferon-stimulated genes mediated by RIG-I-like receptors
文献类型：期刊论文
| 作者 | Chen, Wen Qin1,2; Hu, Yi Wei1,3; Zou, Peng Fei1; Ren, Shi Si1,3; Nie, Pin1; Chang, Ming Xian1 |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| 出版日期 | 2015-03-01 |
| 卷号 | 49期号:1页码:19-30 |
| 关键词 | Zebrafish MAVS variants RIG-I-like receptors Antiviral activity SVCV |
| ISSN号 | 0145-305X |
| 通讯作者 | Chang, MX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China. |
| 英文摘要 | The mitochondrial antiviral signaling protein (MAVS) plays a key role in the signal transduction of RIG-I-like receptors (RLRs)-mediated antiviral response. In the present study, zebrafish MAVS transcript variants, namely MAVS_tv1 and MAVS_tv2, were cloned from zebrafish embryos. The putative MAVS_tv1 protein (full length form) contains an N-terminal CARD domain, a central proline region, and a C-terminal transmembrane domain (TM). MAVS_tv2 is generated by a 190 bp intron fragment insertion. The putative MAVS_tv2 protein lacked TM domain due to a frame shift, with the N-terminal 303 aa residues identical to MAVS_tv1, and no sequence homology for the C-terminal 41 aa residues. Real-time PCR showed that the expression of MAVS_tv1 in ZF4 cells was higher than that of MAVS_tv2, and MAVS variants were induced by Edwardsiella tarda and SVCV infection during the early time points of infection, whereas MAVS_tv1 unchanged or MAVS_tv2 decreased at a later time point after the infection, respectively. Overexpression of MAVS_tv1 and MAVS_tv2 in fish cells conferred antiviral resistance, and activated zebrafish IFN1 and IFN3 promoters. MAVS_tv1 overexpression induced a slow (48 hpf) increased expression of IFNI, mxa, mxb, mxe and RSAD2. In contrast, MAVS tv2 overexpression increased rapidly and transiently the expression of IFN1, IFN2, IFN3, mxc and rsad2 at 6 or 24 hpf. The simultaneous overexpression of MAVS variants and RIG-I in zebrafish embryos led to an accumulative induction of IFNs and IFN-stimulated genes including IFN1, IFN4, mxc, mxe and rsad. Furthermore, MAVS_tv1 cooperated with RIG-I in the accumulation of RIG-I transcript in a positive feedback loop; MAVS_tv2 synergized with MDA5 in the accumulation of MAVS_tv2 transcript. Collectively, these data suggest the molecular mechanisms of fish MAVS variants in antiviral immunity. (C) 2014 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology ; Zoology |
| 研究领域[WOS] | Immunology ; Zoology |
| 关键词[WOS] | NF-KAPPA-B ; ANTIVIRAL SIGNALING PROTEIN ; INNATE IMMUNE-RESPONSES ; ADAPTER PROTEIN ; ACTIVATION ; IDENTIFICATION ; EVOLUTION ; VIRUS ; IPS-1 ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000348889800003 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/23913]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 2.Hubei Vocat Coll Biotechnol, Wuhan 430070, Hubei Province, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Wen Qin,Hu, Yi Wei,Zou, Peng Fei,et al. MAVS splicing variants contribute to the induction of interferon and interferon-stimulated genes mediated by RIG-I-like receptors[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,49(1):19-30. |
| APA | Chen, Wen Qin,Hu, Yi Wei,Zou, Peng Fei,Ren, Shi Si,Nie, Pin,&Chang, Ming Xian.(2015).MAVS splicing variants contribute to the induction of interferon and interferon-stimulated genes mediated by RIG-I-like receptors.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,49(1),19-30. |
| MLA | Chen, Wen Qin,et al."MAVS splicing variants contribute to the induction of interferon and interferon-stimulated genes mediated by RIG-I-like receptors".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 49.1(2015):19-30. |
入库方式： OAI收割
来源：水生生物研究所
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