Fish MAVS is involved in RLR pathway-mediated IFN response
文献类型：期刊论文
| 作者 | Zhang, Jun; Zhang, Yi-Bing; Wu, Min; Wang, Bing; Chen, Chen; Gui, Jian-Fang |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2014-12-01 |
| 卷号 | 41期号:2页码:222-230 |
| 关键词 | Crucian carp Carassius auratus MAVS MITA RLR signaling pathway IFN response |
| ISSN号 | 1050-4648 |
| 通讯作者 | Zhang, YB (reprint author), Chinese Acad Sci, Univ Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| 英文摘要 | Mammalian mitochondrial antiviral signaling protein (MAVS) is an essential adapter involved in retinoic acid-inducible gene-I (RIG-I)-like receptor (RLR)-triggered interferon (IFN) antiviral immunity. Fish MAVS homologues have been identified in several fish species; however, the signaling pathway involving fish MAVS-mediated IFN response remains to be investigated. In the present study, we report identification of a fish MAVS orthologue from crucian carp Carassius auratus blastulae embryonic (CAB) cells and its function role in fish RLR signaling. Crucian carp MAVS is constitutively expressed in CAB cells and is not transcriptionally induced by cytosolic poly (I:C) and IFN. Overexpression of crucian carp MAVS results in activation of fish IFN promoter and ISRE-containing promoter as well as transcriptional expression of IFN and ISGs including PKR and Mx1, which is impaired by functional blockade of signaling molecules TBK1 and IRF3/7. Either cytosolic poly (I:C)-induced or RIG-I-induced IFN response is attenuated by functional blockade of crucian carp MAVS. These results together indicate that fish MAVS contributes to IFN antiviral immunity downstream of cytosolic poly (I:C) and RIG-I and upstream of TBK1 and IRF3/7. Moreover, we provide evidence that apart from crucian carp MAVS, crucian carp MITA is also involved in cytosolic poly (I:C)- and RIG-I-induced IFN response. (C) 2014 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | INTERFERON ANTIVIRAL RESPONSE ; INNATE IMMUNE-RESPONSES ; I-LIKE RECEPTORS ; NF-KAPPA-B ; RIG-I ; CARASSIUS-AURATUS ; ADAPTER PROTEIN ; FUNCTIONAL-CHARACTERIZATION ; SIGNALING PROTEIN ; TELEOST FISH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000347017500013 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/23920]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Univ Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jun,Zhang, Yi-Bing,Wu, Min,et al. Fish MAVS is involved in RLR pathway-mediated IFN response[J]. FISH & SHELLFISH IMMUNOLOGY,2014,41(2):222-230. |
| APA | Zhang, Jun,Zhang, Yi-Bing,Wu, Min,Wang, Bing,Chen, Chen,&Gui, Jian-Fang.(2014).Fish MAVS is involved in RLR pathway-mediated IFN response.FISH & SHELLFISH IMMUNOLOGY,41(2),222-230. |
| MLA | Zhang, Jun,et al."Fish MAVS is involved in RLR pathway-mediated IFN response".FISH & SHELLFISH IMMUNOLOGY 41.2(2014):222-230. |
入库方式： OAI收割
来源：水生生物研究所
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