Cloning of Interleukin-10 from African Clawed Frog (Xenopus tropicalis), with the Finding of IL-19/20 Homologue in the IL-10 Locus
文献类型：期刊论文
| 作者 | Qi, Zhitao1,2; Zhang, Qihuan2; Wang, Zisheng1; Zhao, Weihong1,2; Gao, Qian3 |
| 刊名 | JOURNAL OF IMMUNOLOGY RESEARCH
 |
| 出版日期 | 2015 |
| 期号 | * |
| ISSN号 | 2314-8861 |
| 通讯作者 | Qi, ZT (reprint author), Yancheng Inst Technol, Dept Ocean Technol, Key Lab Aquaculture & Ecol Coastal Pool Jiangsu P, Yancheng 224051, Jiangsu, Peoples R China. |
| 英文摘要 | Interleukin-10 (IL-10) is a pleiotropic cytokine that plays an important role in immune system. In the present study, the IL-10 gene of African clawed frog (Xenopus tropicalis) was first cloned, and its expression pattern and 3D structure were also analyzed. The frog IL-10 mRNA encoded 172 amino acids which possessed several conserved features found in IL-10s from other species, including five-exon/four-intron genomic structure, conserved four cysteine residues, IL-10 family motif, and six alpha-helices. Real-time PCR showed that frog IL-10 mRNA was ubiquitous expressed in all examined tissues, highly in some immune related tissues including kidney, spleen, and intestine and lowly in heart, stomach, and liver. The frog IL-10 mRNA was upregulated at 24 h after LPS stimulation, indicating that it plays a part in the host immune response to bacterial infection. Another IL, termed as IL-20, was identified from the frog IL-10 locus, which might be the homologue of mammalian IL-19/20 according to the analysis results of the phylogenetic tree and the sequence identities. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology |
| 研究领域[WOS] | Immunology |
| 关键词[WOS] | CRYSTAL-STRUCTURE ; PREDICTION ; CYTOKINE ; GENE ; IDENTIFICATION ; EXPRESSION ; IFN ; ALIGNMENT ; SEQUENCE ; REVEALS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000350149300001 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/23926]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Yancheng Inst Technol, Dept Ocean Technol, Key Lab Aquaculture & Ecol Coastal Pool Jiangsu P, Yancheng 224051, Jiangsu, Peoples R China 2.Yancheng Inst Technol, Chem & Biol Engn Coll, Cent Lab Biol, Yancheng 224051, Jiangsu, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430070, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Zhitao,Zhang, Qihuan,Wang, Zisheng,et al. Cloning of Interleukin-10 from African Clawed Frog (Xenopus tropicalis), with the Finding of IL-19/20 Homologue in the IL-10 Locus[J]. JOURNAL OF IMMUNOLOGY RESEARCH,2015(*). |
| APA | Qi, Zhitao,Zhang, Qihuan,Wang, Zisheng,Zhao, Weihong,&Gao, Qian.(2015).Cloning of Interleukin-10 from African Clawed Frog (Xenopus tropicalis), with the Finding of IL-19/20 Homologue in the IL-10 Locus.JOURNAL OF IMMUNOLOGY RESEARCH(*). |
| MLA | Qi, Zhitao,et al."Cloning of Interleukin-10 from African Clawed Frog (Xenopus tropicalis), with the Finding of IL-19/20 Homologue in the IL-10 Locus".JOURNAL OF IMMUNOLOGY RESEARCH .*(2015). |
入库方式： OAI收割
来源：水生生物研究所
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