A N-15 CPMG relaxation dispersion experiment more resistant to resonance offset and pulse imperfection
文献类型：期刊论文
| 作者 | Jiang, Bin1,2 ; Yu, Binhan1; Zhang, Xu2; Liu, Maili2; Yang, Daiwen1
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| 刊名 | JOURNAL OF MAGNETIC RESONANCE
 |
| 出版日期 | 2015-08-01 |
| 卷号 | 257页码:1-7 |
| 关键词 | Protein dynamics Relaxation dispersion CPMG Conformational exchange |
| 英文摘要 | Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion is a powerful NMR method to study protein dynamics on the microsecond-millisecond time scale. J-coupling, resonance offset, radio frequency field inhomogeneity, and pulse imperfection often introduce systematic errors into the measured transverse relaxation rates. Here we proposed a modified continuous wave decoupling CPMG experiment, which is more unaffected by resonance offset and pulse imperfection. We found that it is unnecessary to match the decoupling field strength with the delay between CPMG refocusing pulses, provided that decoupling field is strong enough. The performance of the scheme proposed here was shown by simulations and further demonstrated experimentally on a fatty acid binding protein. (C) 2015 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology |
| 类目[WOS] | Biochemical Research Methods ; Physics, Atomic, Molecular & Chemical ; Spectroscopy |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Physics ; Spectroscopy |
| 关键词[WOS] | CHARACTERIZING CHEMICAL-EXCHANGE ; NMR-SPECTROSCOPY ; MAGNETIC-RESONANCE ; DYNAMICS ; PROTEIN ; MOTIONS ; ARTIFACTS ; SEQUENCE ; LIQUIDS ; SYSTEMS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000358339700001 |
| 公开日期 | 2015-09-15 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/7825]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore 2.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Bin,Yu, Binhan,Zhang, Xu,et al. A N-15 CPMG relaxation dispersion experiment more resistant to resonance offset and pulse imperfection[J]. JOURNAL OF MAGNETIC RESONANCE,2015,257:1-7. |
| APA | Jiang, Bin,Yu, Binhan,Zhang, Xu,Liu, Maili,&Yang, Daiwen.(2015).A N-15 CPMG relaxation dispersion experiment more resistant to resonance offset and pulse imperfection.JOURNAL OF MAGNETIC RESONANCE,257,1-7. |
| MLA | Jiang, Bin,et al."A N-15 CPMG relaxation dispersion experiment more resistant to resonance offset and pulse imperfection".JOURNAL OF MAGNETIC RESONANCE 257(2015):1-7. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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