Direct determination of amino acids and off-line elimination of carbohydrates in tobacco and tobacco feed liquid samples
文献类型：期刊论文
| 作者 | Yuan-Yuan, Pan ; Lina, Liang ; Ya-Qi, Cai ; Shi-Fen, Mon |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2007-12 |
| 卷号 | 35期号:12页码:1761-1766 |
| 关键词 | amino acids high performance anion exchange chromatography integrated pulsed aperometric detection carbohydrates elimination tobacco tobacco feed liquid |
| 英文摘要 | A method was established for the direct determination of amino acids in tobacco and tobacco feed liquid by high performance anion exchange chromatography with integrated pulsed amperometric detection (HPAEC-IPAD). Using an off-line method, high concentrations of carbohydrates which can significantly interfere with amino acids determination were eliminated, including glucose, fructose, sucrose, oligosaccharides, and so on. The separation was carried out on an AminoPac PA10 anion-exchange column with NaOH/ NaAc gradient eluent at a flow rate of 0.25 mL/min. The determination was performed with integrated pulsed amperometric detector. The recoveries were in the range of 76% - 105%. The method can be an efficient tool for the interfere elimination of high concentration of carbohydrates in samples such as tobacco and tobacco feed liquid, and accurate determination of amino acids. |
| WOS记录号 | WOS:000253934500015 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/22445]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Yuan-Yuan, Pan,Lina, Liang,Ya-Qi, Cai,et al. Direct determination of amino acids and off-line elimination of carbohydrates in tobacco and tobacco feed liquid samples[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2007,35(12):1761-1766. |
| APA | Yuan-Yuan, Pan,Lina, Liang,Ya-Qi, Cai,&Shi-Fen, Mon.(2007).Direct determination of amino acids and off-line elimination of carbohydrates in tobacco and tobacco feed liquid samples.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,35(12),1761-1766. |
| MLA | Yuan-Yuan, Pan,et al."Direct determination of amino acids and off-line elimination of carbohydrates in tobacco and tobacco feed liquid samples".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 35.12(2007):1761-1766. |
入库方式： OAI收割
来源：生态环境研究中心
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