Mechanisms involved in ceramide-induced cell cycle arrest in human hepatocarcinoma cells
文献类型：期刊论文
| 作者 | Wang, Jing ; Lv, Xiao-Wen ; Shi, Jie-Ping ; Hu, Xiao-Song |
| 刊名 | WORLD JOURNAL OF GASTROENTEROLOGY
 |
| 出版日期 | 2007-02-21 |
| 卷号 | 13期号:7页码:1129-1134 |
| 关键词 | ceramide cell cycle arrest human hepatocarcinoma cells p21 CyclinD1 CDK7 PPAR gamma ERK |
| 英文摘要 | AIM: To investigate the effect of ceramide on the cell cycle in human hepatocarcinoma Bel7402 cells. Possible molecular mechanisms were explored. METHODS: [3- (4, 5)-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay, plasmid transfection, reporter assay, FACS and Western blotting analyses were employed to investigate the effect and the related molecular mechanisms of C2-ceramide on the cell cycle of Bel7402 cells. RESULTS: C2-ceramide was found to inhibit the growth of Bel7402 cells by inducing cell cycle arrest. During the process, the expression of p21 protein increased, while that of cyclinD1, phospho-ERK1/2 and c-myc decreased. Furthermore, the level of CDK7 was downregulated, while the transcriptional activity of PPAR gamma was upregulated. Addition of GW9662, which is a PPAR gamma specific antagonist, could reserve the modulation action on CDK7. CONCLUSION: Our results support the hypothesis that cell cycle arrest induced by C2-ceramide may be mediated via accumulation of p21 and reduction of cyclinD1 and CDK7, at least partly, through PPAR gamma activation. The ERK signaling pathway was involved in this process. (c) 2007 The WIG Press. All rights reserved. |
| WOS记录号 | WOS:000245221200023 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/22476]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Jing,Lv, Xiao-Wen,Shi, Jie-Ping,et al. Mechanisms involved in ceramide-induced cell cycle arrest in human hepatocarcinoma cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2007,13(7):1129-1134. |
| APA | Wang, Jing,Lv, Xiao-Wen,Shi, Jie-Ping,&Hu, Xiao-Song.(2007).Mechanisms involved in ceramide-induced cell cycle arrest in human hepatocarcinoma cells.WORLD JOURNAL OF GASTROENTEROLOGY,13(7),1129-1134. |
| MLA | Wang, Jing,et al."Mechanisms involved in ceramide-induced cell cycle arrest in human hepatocarcinoma cells".WORLD JOURNAL OF GASTROENTEROLOGY 13.7(2007):1129-1134. |
入库方式： OAI收割
来源：生态环境研究中心
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