Microplate chemiluminescence enzyme immunoassay for the quantitative analysis of free prostate-specific antigen in human serum
文献类型：期刊论文
| 作者 | Shi Gen ; Tang Bao-Jun ; Wang Xu ; Zhao Li-Xia ; Lin Jin-Ming |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2007-11 |
| 卷号 | 35期号:11页码:1541-1547 |
| 关键词 | free-prostate-specific antigen prostate cancer chemiluminescence enzyme immunoassay tumor marker |
| 英文摘要 | A microplate chemiluminescence enzyme immonoassay with high sensitivity for the quantitative analysis of free prostate specific antigen (f-PSA) in human serum was established. The immunoassay utilized alkaline phosphatase (ALP) as the labeled enzyme, and the highly sensitive ALP-AMPPD (4-methoxy-4-(3"-phosphate-phenyl)-spiro-(1, 2-dioxetane-3, 2'-adamantane)) chemiluminescence reaction as the detection system. Several physicochemical parameters such as incubation time, immunoreaction sequence, and detection time were studied and optimized. A one-step sandwiched reaction mode was applied with incubation at 37 degrees C for 2 h and chemiluminescent detection with 50 mu L AMPPD for 30-80 min. The linear range of the proposed method for f-PSA was 0.15-20 mu g/L with r = 0.998. The detection limit was 0. 0 1 mu g/L. Inter-assay and intra-assay RSD were both below 7%. The recovery was 88%-108%. The cross-reactivity of six normal tumor markers in human serum, including CA50, CA125, CA15-3, CA242, CEA and AFP, was studied. It was shown that this method exhibited high specificity to f-PSA. The stability of the method was evaluated by identifying its analytical performance after stored at 4 degrees C and 37 degrees C for 3, 5, 7 days with RSD lower than 6% and correlation coefficient higher than 0. 998, which showed satisfied reliability and stability. Compared with CLIA kit purchased from Monobind. Inc. USA, there was a good correlation between the two methods. The proposed method has shown great potential in the clinical analysis of f-PSA for prostate cancer diagnosis, as well as in the commercial kits development. |
| WOS记录号 | WOS:000252811700001 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/22480]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Shi Gen,Tang Bao-Jun,Wang Xu,et al. Microplate chemiluminescence enzyme immunoassay for the quantitative analysis of free prostate-specific antigen in human serum[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2007,35(11):1541-1547. |
| APA | Shi Gen,Tang Bao-Jun,Wang Xu,Zhao Li-Xia,&Lin Jin-Ming.(2007).Microplate chemiluminescence enzyme immunoassay for the quantitative analysis of free prostate-specific antigen in human serum.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,35(11),1541-1547. |
| MLA | Shi Gen,et al."Microplate chemiluminescence enzyme immunoassay for the quantitative analysis of free prostate-specific antigen in human serum".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 35.11(2007):1541-1547. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。