Assessment of the relative toxicity of Cu2+ by measuring structural changes of supercoiled DNA
文献类型：期刊论文
| 作者 | Pan, Gang ; Chang, Guohua ; Chen, Hao ; Giusti, Lorenzo |
| 刊名 | ENVIRONMENTAL POLLUTION
 |
| 出版日期 | 2007-07 |
| 卷号 | 148期号:2页码:477-482 |
| 关键词 | ecotoxicology relative toxicity copper shape changes of supercoiled DNA |
| 英文摘要 | A method for the measurement of the relative toxicity of Cu2+ in aquatic environments is proposed. It is based on the quantitative measurement on the shape change of the supercoiled DNA after it is contacted with different levels of Cu2+ for various time intervals. In the absence of any redox reagents, all supercoiled DNA degraded into other forms of DNA after 24 h incubation in the presence of 5.13 x 10(-3), 5.08 x 10(-4) and 5.35 x 10(-5) mol/L Cu2+. At a lower Cu2+ concentration (10(-6) mol/L), 44% of supercoiled DNA retained its original supercoiled form after 24 h, and 29% after 48 h. The concentration of RC50, i.e. concentration of pollutants at which 50% of the supercoiled DNA was relaxed compared to control samples, can be obtained from the does-response curves at different exposure time, which may provide a rapid and convenient approach to assess the relative toxicity of environmental pollutants. (c) 2006 Elsevier Ltd. All rights reserved. |
| WOS记录号 | WOS:000247541100011 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/22538]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Pan, Gang,Chang, Guohua,Chen, Hao,et al. Assessment of the relative toxicity of Cu2+ by measuring structural changes of supercoiled DNA[J]. ENVIRONMENTAL POLLUTION,2007,148(2):477-482. |
| APA | Pan, Gang,Chang, Guohua,Chen, Hao,&Giusti, Lorenzo.(2007).Assessment of the relative toxicity of Cu2+ by measuring structural changes of supercoiled DNA.ENVIRONMENTAL POLLUTION,148(2),477-482. |
| MLA | Pan, Gang,et al."Assessment of the relative toxicity of Cu2+ by measuring structural changes of supercoiled DNA".ENVIRONMENTAL POLLUTION 148.2(2007):477-482. |
入库方式： OAI收割
来源：生态环境研究中心
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