Moderate PEGylation of the carrier protein improves the polysaccharide-specific immunogenicity of meningococcal group A polysaccharide conjugate vaccine
文献类型：期刊论文
| 作者 | Zhang, Tingting1,2; Yu, Weili2; Wang, Yanfei1; Hu, Tao2 |
| 刊名 | VACCINE
 |
| 出版日期 | 2015-06-22 |
| 卷号 | 33期号:28页码:3208-3214 |
| 关键词 | Meningococcal polysaccharide Conjugate vaccine PEGylation Oligomerization |
| ISSN号 | 0264-410X |
| 英文摘要 | Neisseria meningitidis can cause severe and fulminant diseases such as meningitis. Meningococcal capsular polysaccharide (PS) is a key virulence determinant that is not able to induce immunological memory. Conjugation of PS to a carrier protein can significantly increase the immunogenicity of PS and induce immunological memory. Due to the classically described carrier-induced epitopic suppression (CIES) mechanisms, a strong immune response against the carrier protein could suppress the immune response to PS after coadministration of free carrier protein with the conjugate vaccine. However, it was not clear whether suppressing Or enhancing the protein-specific immunogenicity could improve the PS-specific immunogenicity of the conjugate vaccine. Thus, moderate PEGylation, extensive PEGylation and oligomerization were used to regulate the immunogenicity of tetanus toxoid (TT) in the conjugate vaccine (PS-TT). Moderate PEGylation led to a 2.7-fold increase in the PS-specific IgG titers elicited by PS-IT. In contrast, extensive PEGylation and oligomerization of TT led to 1.4-fold and 1.6-fold decrease in the PS-specific IgG titers elicited by PS-TT, respectively. The PS-specific immunogenicity of PS-TT can be increased by moderate PEGylation through mild suppression of the TT-specific immunogenicity. The PS-specific immunogenicity of PS-TT was decreased through significant suppression or enhancement of the TT-specific immunogenicity. Thus, our study contributes to understand the CIES mechanisms and improve the PS-specific immunogenicity of a meningococcal PS conjugate vaccine. (C) 2015 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology ; Medicine, Research & Experimental |
| 研究领域[WOS] | Immunology ; Research & Experimental Medicine |
| 关键词[WOS] | ANTIBODY-RESPONSE ; SPACER ARM ; PEG |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000357235600010 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/19391]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Tingting,Yu, Weili,Wang, Yanfei,et al. Moderate PEGylation of the carrier protein improves the polysaccharide-specific immunogenicity of meningococcal group A polysaccharide conjugate vaccine[J]. VACCINE,2015,33(28):3208-3214. |
| APA | Zhang, Tingting,Yu, Weili,Wang, Yanfei,&Hu, Tao.(2015).Moderate PEGylation of the carrier protein improves the polysaccharide-specific immunogenicity of meningococcal group A polysaccharide conjugate vaccine.VACCINE,33(28),3208-3214. |
| MLA | Zhang, Tingting,et al."Moderate PEGylation of the carrier protein improves the polysaccharide-specific immunogenicity of meningococcal group A polysaccharide conjugate vaccine".VACCINE 33.28(2015):3208-3214. |
入库方式： OAI收割
来源：过程工程研究所
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