Molecular specificity and detection for Pseudanabaena (cyanobacteria) species based on rbcLX sequences
文献类型：期刊论文
| 作者 | Zhu, Mengling1,2; Yu, Gongliang3; Song, Gaofei3; Chang, Jianbo1,2; Wan, Chengyan1,2; Li, Renhui3 |
| 刊名 | BIOCHEMICAL SYSTEMATICS AND ECOLOGY
 |
| 出版日期 | 2015-06-01 |
| 卷号 | 60页码:110-115 |
| 关键词 | rbcLX Cyanobacteria Molecular detection Pseudanabaena Realtime-PCR |
| ISSN号 | 0305-1978 |
| 通讯作者 | Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Peoples R China. |
| 英文摘要 | In decades, Pseudanabaena species have been frequently reported as cyanobacterial bloom components. However, these species are always overlooked because they cannot be observed easily owing to their small sizes. This study aimed to characterize Pseudanabaena species and detect them in field waters by developing an effective DNA marker. A region with approximately 600 bp in the rbcLX sequences was specific to Pseudanabaena species alone compared with other cyanobacterial genera. Therefore, new primers specific for Pseudanabaena were generated. By using this molecular tool, seasonal and spatial dynamics of Pseudanabaena populations of six sites in Lushui Reservoir, China were measured from July 2010 to August 2011. Pseudanabaena species existed in the reservoir throughout the year, and cell abundance ranged between 10(4) and 10(6) equivalent cells/L, with the peak at 8.07 x 10(6) equivalent cells/L The seasonal dynamics pattern of Pseudanabaena was similar to that of Microcystis but showed a slight lag at the transitional points of water temperature in the reservoir. (C) 2015 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology |
| 关键词[WOS] | STRAINS ; QUANTIFICATION ; OSCILLATORIALES ; PHYTOPLANKTON ; SYSTEMS ; CHINA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000357546800017 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/25752]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.Minist Water Resources, Inst Hydroecol, Key Lab Ecol Impacts Hydraul Projects & Restorat, Wuhan 430079, Peoples R China 2.Chinese Acad Sci, Wuhan 430079, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Mengling,Yu, Gongliang,Song, Gaofei,et al. Molecular specificity and detection for Pseudanabaena (cyanobacteria) species based on rbcLX sequences[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2015,60:110-115. |
| APA | Zhu, Mengling,Yu, Gongliang,Song, Gaofei,Chang, Jianbo,Wan, Chengyan,&Li, Renhui.(2015).Molecular specificity and detection for Pseudanabaena (cyanobacteria) species based on rbcLX sequences.BIOCHEMICAL SYSTEMATICS AND ECOLOGY,60,110-115. |
| MLA | Zhu, Mengling,et al."Molecular specificity and detection for Pseudanabaena (cyanobacteria) species based on rbcLX sequences".BIOCHEMICAL SYSTEMATICS AND ECOLOGY 60(2015):110-115. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。