Morphological alterations and acetylcholinesterase and monoamine oxidase inhibition in liver of zebrafish exposed to Aphanizomenon flos-aquae DC-1 aphantoxins
文献类型：期刊论文
| 作者 | Zhang, De Lu1; Zhang, Jing2; Hu, Chun Xiang3; Wang, Gao Hong3; Li, Dun Hai3; Liu, Yong Ding3 |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2014-12-01 |
| 卷号 | 157页码:215-224 |
| 关键词 | Cyanobacterial neurotoxin Zebrafish liver Morphological alteration Acetylcholinesterase Monoamine oxidase Neurotoxicity |
| ISSN号 | 0166-445X |
| 通讯作者 | Hu, CX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China. |
| 英文摘要 | Aphanizomenon flos-aquae is a cyanobacterium that produces neurotoxins or paralytic shellfish poisons (PSPs) called aphantoxins, which present threats to environmental safety and human health via eutrophication of water bodies worldwide. Although the molecular mechanisms of this neurotoxin have been studied, many questions remain unsolved, including those relating to in vivo hepatic neurotransmitter inactivation, physiological detoxification and histological and ultrastructural alterations. Aphantoxins extracted from the natural strain of A. flos-aquae DC-1 were analyzed by high-performance liquid chromatography. The main components were gonyautoxins 1 and 5 (GTX1, GTX5) and neosaxitoxin (neoSTX), which comprised 34.04%, 21.28%, and 12.77% respectively. Zebrafish (Danio rerio) were exposed intraperitoneally to 5.3 or 7.61 mu g STX equivalents (eq)/kg (low and high doses, respectively) of A. flos-aquae DC-1 aphantoxins. Morphological alterations and changes in neurotransmitter conduction functions of acetylcholinesterase (AChE) and monoamine oxidase (MAO) in zebrafish liver were detected at different time points 1-24 h post-exposure. Aphantoxin significantly enhanced hepatic alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and histological and ultrastructural damage in zebrafish liver at 3-12 h post-exposure. Toxin exposure increased the reactive oxygen species content and reduced total antioxidative capacity in zebrafish liver, suggesting oxidative stress. AChE and MAO activities were significantly inhibited, suggesting neurotransmitter inactivation/conduction function abnormalities in zebrafish liver. All alterations were dose- and time-dependent. Overall, the results indicate that aphantoxins/PSPs induce oxidative stress through inhibition of AChE and MAO activities, leading to neurotoxicity in zebrafish liver. The above parameters may be useful as bioindicators for investigating aphantoxins/PSPs and cyanobacterial blooms in nature. (C) 2014 Published by Elsevier B.V. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Marine & Freshwater Biology ; Toxicology |
| 研究领域[WOS] | Marine & Freshwater Biology ; Toxicology |
| 关键词[WOS] | SHELLFISH POISONING TOXINS ; DICENTRARCHUS-LABRAX L. ; CARP CYPRINUS-CARPIO ; CARASSIUS-AURATUS ; BIOCHEMICAL PARAMETERS ; CYANOBACTERIAL BLOOM ; LIPID-PEROXIDATION ; OXIDATIVE STRESS ; GRUZA RESERVOIR ; SAUDI-ARABIA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000346547800023 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/25782]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, Dept Lifesci & Biotechnol, Wuhan 430070, Peoples R China 2.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, De Lu,Zhang, Jing,Hu, Chun Xiang,et al. Morphological alterations and acetylcholinesterase and monoamine oxidase inhibition in liver of zebrafish exposed to Aphanizomenon flos-aquae DC-1 aphantoxins[J]. AQUATIC TOXICOLOGY,2014,157:215-224. |
| APA | Zhang, De Lu,Zhang, Jing,Hu, Chun Xiang,Wang, Gao Hong,Li, Dun Hai,&Liu, Yong Ding.(2014).Morphological alterations and acetylcholinesterase and monoamine oxidase inhibition in liver of zebrafish exposed to Aphanizomenon flos-aquae DC-1 aphantoxins.AQUATIC TOXICOLOGY,157,215-224. |
| MLA | Zhang, De Lu,et al."Morphological alterations and acetylcholinesterase and monoamine oxidase inhibition in liver of zebrafish exposed to Aphanizomenon flos-aquae DC-1 aphantoxins".AQUATIC TOXICOLOGY 157(2014):215-224. |
入库方式： OAI收割
来源：水生生物研究所
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