Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry
文献类型：期刊论文
| 作者 | Sun HL; Qi C(齐财); Niu Y(牛宇) ; Kang TF(康腾飞); Wei YX; Jin G(靳刚); Dong XZ; Wang CH; Zhu W
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| 刊名 | PLOS ONE
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| 出版日期 | 2015-08-21 |
| 卷号 | 10期号:8页码:e0136253 |
| 通讯作者邮箱 | fsszw@163.com |
| ISSN号 | 1932-6203 |
| 产权排序 | [Sun, Hongliu; Wang, Chunhua] Binzhou Med Univ, Sch Pharmaceut Sci, Yantai, Peoples R China; [Qi, Cai] Chinese Acad Inspect & Quarantine, Inst Equipment Technol, Beijing 3, Peoples R China; [Niu, Yu; Kang, Tengfei; Jin, Gang] Chinese Acad Sci, Inst Mech, Beijing 100080 15, Peoples R China; [Wei, Yongxin] Beijing Inspect & Quarantine Testing Ctr, Food Lab, Beijing 6, Peoples R China; [Dong, Xianzhi] Chinese Acad Sci, Inst Biophys, Beijing 100080 15, Peoples R China; [Zhu, Wei] Shandong Acad Med Sci, Inst Radiat Med, Jinan 18877, Peoples R China |
| 通讯作者 | Zhu, W (reprint author), Shandong Acad Med Sci, Inst Radiat Med, Jinan 18877, Peoples R China. |
| 中文摘要 | Background Cytomegalovirus (CMV) is the most common infectious cause of mental disability in new-borns in developed countries. There is an urgent need to establish an early detection and high-throughput screening method for CMV infection using portable detection devices. Methods An antibody analysis method is reported for the detection and identification of CMV antibodies in serum using a biosensor based on high spatial resolution imaging ellipsometry (BIE). CMV antigen (CMV-3A) was immobilized on silicon wafers and used to capture CMV antibodies in serum. An antibody against human immunoglobulin G (anti-IgG) was used to confirm the IgG antibody against CMV captured by the CMV-3A. Results Our results show that this assay is rapid and specific for the identification of IgG antibody against CMV. Further, patient serum was quantitatively assessed using the standard curve method, and the quantitative results were in agreement with the enzyme-linked immunosorbent assay. The CMV antibody detection sensitivity of BIE reached 0.01 IU/mL. Conclusions This novel biosensor may be a valuable diagnostic tool for analysis of IgG antibody against CMV during CMV infection screening. |
| 分类号 | 二类/Q1 |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1371/journal.pone.0136253 |
| 语种 | 英语 |
| WOS记录号 | WOS:000359926900090 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/55341]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Sun HL,Qi C,Niu Y,et al. Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry[J]. PLOS ONE,2015,10(8):e0136253. |
| APA | Sun HL.,Qi C.,Niu Y.,Kang TF.,Wei YX.,...&Zhu W.(2015).Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry.PLOS ONE,10(8),e0136253. |
| MLA | Sun HL,et al."Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry".PLOS ONE 10.8(2015):e0136253. |
入库方式： OAI收割
来源：力学研究所
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